Label-free biomedical optical imaging and sensing refers to optical measurements performed on biological samples or living organisms without the need for utilizing labeling agents. Label-free imaging of cells in vitro is specifically of interest, since isolated cells are optically transparent and regular bright-field imaging does not present enough imaging contrast. Labeling agents, such as fluorescent dyes or labels using antibodies, can create molecular specificity and enhance contrast but they might interfere with the biological phenomena measured, and thus are not always allowed. In addition, some biological targets do not have suitable labeling agents. In vivo imaging of living organisms, and humans in particular, should be preferably performed without using labeling agents, due to possible hazardous effects induced by these agents.

Optical detection methods for label-free imaging and sensing are typically based on internal contrast mechanisms of the sample; for example, its ability to delay the light interacting with the sample due to refractive index changes, or its ability to create unique optical spectroscopic, auto-fluorescence, and birefringence or acoustic signatures. In addition, the substrate holding the sample during measurement can be used to enhance the detection and monitor of the sample properties via various effects, including Plasmon resonance, total internal reflection, etc. Furthermore, life science tools, such as optogenetics or gene expression methods, can be applied to achieve molecular specificity in living objects.

Label-free imaging and sensing in the nanoscale, including tracking of single molecules, is of high interest as well. Specifically, label-free optical nanoscopy is still considered as an unsolved challenge in this field.

This conference will gather scientists from various disciplines, who are interested in optical imaging and sensing of biological substances without using labeling: physicists and engineers on the one hand, chemists and life scientists performing optical label-free sensing on the other hand. Keynote presentations for 2021 included:
Relevant topics include, but are not limited to: ;
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Conference BO509

Label-free Biomedical Imaging and Sensing (LBIS) 2022

This conference has an open call for papers:
Abstract Due: 11 August 2021
Author Notification: 11 October 2021
Manuscript Due: 29 December 2021
Label-free biomedical optical imaging and sensing refers to optical measurements performed on biological samples or living organisms without the need for utilizing labeling agents. Label-free imaging of cells in vitro is specifically of interest, since isolated cells are optically transparent and regular bright-field imaging does not present enough imaging contrast. Labeling agents, such as fluorescent dyes or labels using antibodies, can create molecular specificity and enhance contrast but they might interfere with the biological phenomena measured, and thus are not always allowed. In addition, some biological targets do not have suitable labeling agents. In vivo imaging of living organisms, and humans in particular, should be preferably performed without using labeling agents, due to possible hazardous effects induced by these agents.

Optical detection methods for label-free imaging and sensing are typically based on internal contrast mechanisms of the sample; for example, its ability to delay the light interacting with the sample due to refractive index changes, or its ability to create unique optical spectroscopic, auto-fluorescence, and birefringence or acoustic signatures. In addition, the substrate holding the sample during measurement can be used to enhance the detection and monitor of the sample properties via various effects, including Plasmon resonance, total internal reflection, etc. Furthermore, life science tools, such as optogenetics or gene expression methods, can be applied to achieve molecular specificity in living objects.

Label-free imaging and sensing in the nanoscale, including tracking of single molecules, is of high interest as well. Specifically, label-free optical nanoscopy is still considered as an unsolved challenge in this field.

This conference will gather scientists from various disciplines, who are interested in optical imaging and sensing of biological substances without using labeling: physicists and engineers on the one hand, chemists and life scientists performing optical label-free sensing on the other hand. Keynote presentations for 2021 included:
  • Stephen A. Boppart, University of Illinois, USA
  • Altug Hatice, EPFL, Switzerland
  • Gabriel Popescu, University of Illinois, USA

Relevant topics include, but are not limited to:
  • Phase imaging (Zernike’s, differential interference contrast (DIC), holography, optical diffraction tomography (ODT), etc.)
  • Coherent Raman spectroscopy techniques (CARS, SRS, etc.)
  • Spontaneous Raman imaging
  • Interferometric and coherence gated imaging (optical coherence tomography, etc.)
  • Polarization and birefringence imaging
  • Dark-field microscopy
  • Brillouin microscopy (spontaneous and stimulated)
  • High harmonic generation and nonlinear imaging and sensing
  • Auto-fluorescence imaging and sensing
  • Hyperspectral imaging and sensing
  • Total internal reflection imaging and sensing
  • Acoustic and photoacoustic imaging
  • Plasmonic sensors
  • Fiber-optics-based label-free bio-detectors
  • Label-free imaging in the nano-scale
  • On-chip implementations of label-free sensors
  • Preclinical, clinical, and life science applications
  • Label-free imaging using optogenetic and gene expression tools.
Conference Chair
Tel Aviv Univ. (Israel)
Conference Chair
Technische Univ. München (Germany)
Program Committee
National Taiwan Univ. (Taiwan)
Program Committee
Adam de la Zerda
Stanford Univ. School of Medicine (United States)
Program Committee
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello" (Italy)
Program Committee
Jochen R. Guck
TU Dresden (Germany)
Program Committee
Univ. of California, Los Angeles (United States)
Program Committee
Univ. at Albany (United States)
Program Committee
Ctr. de Recherche de l'Univ. Laval Robert-Giffard (Canada)
Program Committee
Friedrich-Schiller-Univ. Jena (Germany)
Program Committee
Georgia Institute of Technology & Emory Univ. School of Medicine (United States)
Program Committee
Univ. of Wisconsin-Madison (United States)
Program Committee
Saratov State Univ. (Russian Federation), Tomsk State Univ. (Russian Federation), Institute of Precision Mechanics and Control of the RAS (Russian Federation)
Program Committee
Changchun Institute of Optics, Fine Mechanics and Physics (China)
Program Committee
Virginia Polytechnic Institute and State Univ. (United States)