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The focus of this conference will be on invasive and noninvasive optical methods for the diagnostics and sensing of all types of biological fluids: blood, lymph, saliva, mucus, gastric juice, urine, aqueous humor, semen, etc. both in vitro and in vivo with, for example, point-of-care microfluidic technologies, mobile technology platforms such as cell phones and tablets, and/or wearable photonic technologies.

The techniques to monitor the fluids and optical properties of these fluids will be discussed including: elastic, quasi-elastic, and inelastic (Raman) light scattering, surface enhanced Raman (SERS) techniques, Doppler flowmetry, spectrophotometry, polarimetry, diffraction, holography, speckle, fluorescence, imaging, and related spectroscopic and microscopic techniques. Studies of biological fluid components on cellular and macromolecular levels, as well as nondestructive measurements of analyte content, will be presented. Theoretical and model studies, as well as clinical applications of the developed optical methods and instrumentation, will be outlined. Diagnostics and sensing systems for point-of-care and global health applications are particularly encouraged.

Suggested topics include, but are not limited to, the following areas: ;
In progress – view active session
Conference 11968

Optical Diagnostics and Sensing XXII: Toward Point-of-Care Diagnostics

In person: 24 January 2022
View Session ∨
  • 1: Microscopic Systems for Sensing and Monitoring
  • 2: Paper Fluidic Monitoring and Diagnostic Systems
  • 3: Optical Microfluidic and Well Array Systems
  • 4: Spectral In vivio and Phantom Systems
  • Posters


  • Submissions are accepted through 06-December
  • Notification of acceptance by 20-December

View Call for Papers PDF Flyer
Session 1: Microscopic Systems for Sensing and Monitoring
Author(s): Sebastian Dochow, Jenoptik Optical Systems GmbH (Germany); Bernhard Messerschmidt, Grintech GmbH (Germany); Sven Urban, Jenoptik Optical Systems GmbH (Germany); Herbert Gross, University of Jena, Institute of Applied Physics (Germany)
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For the industrialization of point-of-care applications, the miniaturization of diagnostic microscopes maintaining their performance for a reasonable pricing and a fast time-to-market are the biggest challenges. Providing application-tailored digital microscope subsystems for integration into next-generation devices, they have to meet the needs for image quality, automation and digitalization. With the invention of JENOPTIK SYIONS as a platform for many different applications (e.g. fluorescence, bright field, dark-field, Raman microscopy) a concept is proven to be reliable and flexible on, e.g. size, application-dependent target resolutions or needed field-of views.
Author(s): Kerem Delikoyun, Izmir Institute of Technology (Turkey); Huseyin Cumhur Tekin, Izmir Institute of Technology (Turkey), METU MEMS Center (Turkey)
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Protein detection in clinics is of great importance, however, traditional assays are not suitable for daily use. We present a novel platform that uses lensless holographic microscopy for ultrasensitive detection of proteins based on surface coverage assays. In this scheme, magnetic microbeads used as labels are captured in the microfluidic channel in the presence of target proteins. Then, a holographic image of the microfluidic channel is acquired to count captured microbeads and to correlate with target protein concentrations. All quantification processes are performed autonomously on this portable platform, which enables rapid detection of target proteins down to pg/mL levels.
Author(s): Euan McLeod, Maryam Baker, Colin J. Potter, Weilin Liu, University of Arizona (United States)
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Bead-based assays are traditionally difficult to adapt for high-sensitivity quantitative point-of-care diagnostics. Here we use lensfree optical microscopes with automated image processing to quantitatively sense specific proteins in solution via the agglutination of functionalized beads within a microfluidic chip. Simple protocols and compact and inexpensive readout devices make our approach well-suited for point-of-care diagnostics. We sense interferon gamma, a biomarker of infectious and inflammatory disease, as well as NeutrAvidin. Furthermore, we discuss computational methods for improving the identification of small particles in the lensfree images, including sparsity-promoting regularized reconstruction and vectorial Green’s function modeling based on dipole electromagnetic scattering theory.
Author(s): Viswanath Gorti, Francisco E. Robles, Coulter Dept. of Biomedical Engineering, Georgia Institute of Technology and Emory University (United States)
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Deep Ultraviolet (UV) Microscopy enables high-resolution, label-free molecular imaging. Recently, it has been used as a tool for fast hematology analysis via imaging and classification of blood cells. Here, we present a compact, low-cost deep-UV microscope capable of performing a rapid complete blood count (CBC). We demonstrate the optical performance of our system and compare hematology analysis results with clinical gold standards and blood smears. We show that this technique can serve as a simple point-of-care alternative to modern hematology analyzers.
Author(s): Ashkan Ojaghi, Evelyn Williams, Francisco E. Robles, Georgia Institute of Technology & Emory Univ School of Medicine (United States)
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We demonstrate a label-free approach for hematological assessment of neutropenia using a Polydimethylsiloxane (PDMS) microfluidic device along with deep-ultraviolet microscopy. Our method benefits from the capabilities of PDMS-based microfluidic devices to form a single-cell layer from a small volume of whole blood for quantitative and qualitative analysis of blood cells. We first demonstrate the capabilities of our approach in detection and staging of neutropenia by imaging whole blood obtained from healthy donors as well as severe and moderate neutropenia patients. Lastly, we show the correlation between the absolute neutrophil counts obtained using our approach with those from complete blood counts.
Author(s): Marisa M. Morakis, Nicholas J. Durr, Johns Hopkins University (United States)
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The increasing performance and ubiquity of mobile phone cameras has led to several emerging opportunities for global health and point-of-care diagnostics. While previous work showed that reverse lens mobile phone capillaroscopy can visualize optical absorption gaps in nailfold capillaries for neutropenia screening, improved resolution and oblique illumination for phase contrast imaging is required to more directly visualize both red and white blood cells. We present a design for a reverse lens mobile phone capillaroscope with a magnification of 1.65x and a resolution of 1.5 μm, whereas the previous design yielded a resolution of 1.91 μm.
Session 2: Paper Fluidic Monitoring and Diagnostic Systems
Author(s): Anna S. Rourke, Vanderbilt Unviersity (United States); Alec Walter, Vanderbilt University (United States); Anita Mahadevan-Jansen, Andrea Locke, Vanderbilt Unviersity (United States)
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Coupling of surface-enhanced Raman spectroscopy (SERS) with the coffee ring effect can overcome the poor reproducibility typically seen when using SERS. In this study, we developed a nitrocellulose membrane paper-based substrate for coffee ring enhanced SERS, which was highly hydrophobic and produced consistent coffee rings. After optimization of solution parameters including gold nanoparticle concentration and solvent, this platform demonstrated high enhancement and low variability using Malachite Green Isothiocyanate and Moraxella catarrhalis. This substrate has the potential to increase the usability and implementation of SERS by overcoming intrinsic limitations and is more accessible than current substrates.
Author(s): Nandita Chaturvedi, Texas A&M University (United States); Samuel Mabbott, Department of Biomedical Engineering (United States), Center for Remote Health and Technologies & Systems, Texas A&M Engineering Experiment Station (United States); Gerard L. Coté, Department of Biomedical Engineering (United States), Center for Remote Health and Technologies & Systems, Texas A&M Engineering Experiment Station (United States), Department of Electrical and Computer Engineering, Texas A&M University (United States)
Author(s): Damber Thapa, Nakisa Samadi, Nima Tabatabaei, York Univ (Canada)
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Antibodies that are produced following infection due to the SARS-CoV-2 virus or vaccination are critical for monitoring the immune response of an individual or the impact of the vaccine over time. As vaccines become available, there is a need for rapid, accurate, and low-cost point-of-care tools for monitoring the effectiveness of the vaccines over time at the population level. Here, we report the efficiency of a handheld point-of-care thermo-photonic device for quantifying anti-SARS-CoV-2 antibodies in humanized control positive solution. Results showed that the imager in conjunction with rapid diagnostic tests (RDT) can detect and quantify antibody levels within clinically relevant range and with a limit of detection of 0.1 µg/ml.
Author(s): Alice Iles, Panagiotis Galanis, Collin Sones, Robert Eason, University of Southampton (United Kingdom)
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Lateral flow devices (LFDs) are widely used point-of-care (POC) diagnostics. The basic LFD design remains largely unchanged since their first development and this limits their use in clinical applications due to lack of sensitivity. To enhance this, we report the use of laser-patterned geometric control barriers, in the form of a constriction, that leads to a slower flow rate and smaller test zone area. This high sensitivity LFD (HS-LFD) achieved 62% increase in test line colour intensity for the detection of procalcitonin (PCT) and reduced the LOD from 10 ng/ml to 1 ng/ml with contrived human samples
Session 3: Optical Microfluidic and Well Array Systems
Author(s): Esmat Z. Ahmad, Polytechnique Montréal (Canada), Centre de recherche du Centre hospitalier de l’Université de Montréal (Canada), Institut du cancer de Montréal (Canada)
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We present the preliminary results of a single-point system applied to saliva analysis using a Surface Enhanced Raman Spectroscopy (SERS) based approach. The methodology interrogates macroscopic droplets of saliva with an emphasis on the inter-droplet variability of the sample, the quantification of the repeatability across individuals and the overall reliability of the approach compared to a gold standard commercial Raman microscopic system. The overall repeatability of the methodology is tested on a large cohort of 500 different individuals to investigate the different confounding factors involved in saliva-based clinical Raman diagnostics and the compatibility of the approach with machine learning (ML) based analysis.
Author(s): Philip Measor, Whitworth University (United States)
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A 3D printed microarray device towards COVID 19 (SARS-COV-2) detection with a limit-of-detection of <190 nM was demonstrated. An array of 1,166 microwells, 116 x 116 µm in size, were 3D printed and synthetic targets and probes specific to COVID-19 spike-proteins were detected to demonstrate a device towards point-of-care COVID-19 detection.
Author(s): Gabriel P. Lachance, Élodie Boisselier, Amine Miled, Univ Laval (Canada)
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In this work, we show a device capable of measuring neurotransmitters in a liquid sample using functionalized ultrastable gold nanoparticles. Using the colorimetric approach, the plasmonic resonance band of nanoparticles shifts when they interact with neurotransmitter enabling their measurements. The functionalization of the nanoparticles with dopamine-specific aptamer increases their response and selectivity to it. Using ultrastable gold nanoparticles provides the potential to expose them to harsh conditions without agglomeration, giving the potential to reuse the nanoparticles for several sensing cycles. Altogether, the system showed that the absorption spectrum of a nanoparticles sample with a resolution of 1 nm can be extracted autonomously using this system.
Author(s): Hua En Chueh, Chen Han Huang, National Central University (Taiwan)
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Oral squamous cell carcinoma (OSCC) accounts for about 90% of oral cancer, and it remains a major cause of patient morbidity and mortality. Time-consuming and inconvenient are two main problems of current detection methods like ELISA or PCR. Here, we established an integrated microfluidic system for functional immunoassay, presenting a rapid optical measurement for Interleukin 6 (IL-6) and IL-8, which are proven to be useful biomarkers. Furthermore, concentration details can be obtained by converting light absorption intensity of OSCC biomarkers. The results show that this portable system has the potential to assist the diagnosis of OSCC at an early stage.
Author(s): Nityanand Kumawat, Priyamvada Venugopalan, Sunil Kumar, New York University Abu Dhabi (United Arab Emirates)
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A low-cost microfluidic chip is developed for bio-sensing and quality control applications. The sensor chip was developed on a compact disc (CD) substrate that has grating structures on it. The high quality gratings on the CD substrate were used to create the diffraction orders in both the transmission and the reflection on illumination by a laser beam. The higher order diffraction orders become extinct as the grating substrate is rotated and the intensity becomes zero at the specific angle of incidence. For the current study, the first order diffraction order was used before the extinction angle giving higher sensitivity.
Session 4: Spectral In vivio and Phantom Systems
Author(s): Jesse N. Fine, Michael McShane, Gerard Coté, Texas A&M University (United States)
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Devices for remote monitoring of a patient’s health can help to provide better access to healthcare, particularly for to underserved communities. Here, we present the development of an in -vitro testing system towards for use with optical remote monitoring devices that usesuch as photoplethysmography (PPG) to measure cardiovascular parameters. This system includes three layer optical phantoms to mimic tissue optical properties, a blood phantom and a pump system to replicate the PPG waveform in vitro. In the this work, we validated the optical properties and size characteristics of the optical phantoms and illustrated the impact of skin tone can have on the amplitude and signal strength of a PPG waveform.
Author(s): Alessandro Marone, Nisha Maheshwari, Hyun K. Kim, New York University (United States); Danielle R. Bajakian, Columbia University Medical Center (United States); Andreas H. Hielscher, New York University (United States)
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We report on the potential of dynamic vascular optical imaging to predict the long-term outcome of a surgical interventions to improve blood flow to in diabetic patients with peripheral artery disease. 16 diabetic patients with no previous history of interventions were enrolled, and DOTI data was collected immediately before and after the intervention. We found a strong correlation between the changes in rise-time of the dynamic signal in response to a cuff and the improvement in blood perfusion 3 months post intervention. A ROC analysis yielded an AUC of 83% with a specificity of 100% and sensitivity of 75%.
Author(s): Nisha Maheshwari, Alessandro Marone, Mirella Altoé, Hyun K. Kim, NYU (United States); Danielle R. Bajakian, Columbia University Irving Medical Center (United States); Andreas H. Hielscher, NYU (United States)
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Early prognosis of ulcer healing can help patients avoid pain and future amputation by alerting physicians to intervention efficacy. The vascular optical tomography imaging system (VOTIS) developed in our laboratory has the potential to address current challenges with ulcer monitoring. In this pilot study, we focused on 10 patients with peripheral arterial disease who had ulcers and surgical intervention. VOTIS measurements were obtained immediately after the intervention, and three weeks afterward. We identified an imaging biomarker with high prognosis classification potential (Sn=86%, Sp=100%, AUC=0.95). If confirmed in a larger clinical study, VOTIS could be used to reliably predict intervention outcome.
Author(s): Andres J. Rodriguez, Tananant Boonya-ananta, Ajmal Ajmal, Florida International University (United States); Ingemar Fredriksson, Tomas Strömberg, Linköping University (Sweden); Jessica C. Ramella-Roman, Florida International University (United States)
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Obesity affects over 40% of the US adult population and increases the risk of diseases such as cardiovascular disease, type-2 diabetes, and many others. Wearable devices have the potential of helping users reduce their obesity levels by facilitating biometric data with users and clinicians. However, wearables depend on optical sensors that may be significantly sensitive to obesity-driven molecular changes in skin composition. Using an SFDS system, we capture skin optical properties at multiple anatomical regions. Our results are validated using a clinical system, EPOS 6000. Analytical and statistical methods are used to determine interactions between measured data and participant demographics.
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The prevalence of heart failure has been increasing worldwide. It is important for patients to assess leg edema regularly, but current assessment methods are not accurate. The objective of this study is to optimize the diffuse reflectance spectroscopy (DRS) system for evaluating leg edema. First, an ideal area to evaluate leg edema was investigated using ultrasound diagnostic equipment. Secondly, optical penetration depth was examined with different source-detector distances. The DRS system optimized by our result obtained correlation between water content and absorbance in leg edema phantom. It was suggested that leg edema could be evaluated quantitatively in DRS system.
Author(s): Rajagopal Srinivasan, ORCA Med, LLC (United States), University of Maryland Emergency Medicine (United States)
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Catheter-associated urinary tract infection (CAUTI) is a serious healthcare problem that plagues those dependent on indwelling urinary catheters. Each CAUTI costs a hospital over $13,000 in unreimbursed care, and the total US economic cost from unreimbursed CAUTI care in 2016 was estimated at $1.7B. Unfortunately, existing tests cannot differentiate actual infection from asymptomatic bacteriuria (ASB), which is common in this population. When existing tests are used indiscriminately on this vulnerable population, they predictably result in unnecessary antibiosis and rapid emergence of antibiotic resistance. In a pilot study, we evaluated an easy-to-use handheld optical device that can quickly analyze bodily fluids through the walls of tubing, without contacting the fluid, for discriminating CAUTI from ASB and negative/normal samples. We found that the device can rapidly predict negative urine culture results and distinguish CAUTI from ASB.
Conference attendees are invited to attend the BiOS poster session. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field. :: :: View poster presentation guidelines and set-up instructions at: :: [{}+{}]
Author(s): Masato Takahashi, Chiba Univ. (Japan); Takeshi Yamaguchi, International University of Health and Welfare (Japan); Keiko OGAWA-OCHIAI, Hiroshima University Hospital (Japan); Norimichi Tsumura, Chiba Univ. (Japan); Norio Iijima, International University of Health and Welfare (Japan)
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In this paper, we report the results of accuracy verification of a measurement method for capturing pulse waves from the sole of rodents using an RGB camera in a non-invasive manner. In order to acquire biometric data such as pulse waves from animals, a non-invasive method is required to minimize the impact on the animal. We experimented with verifying the accuracy using an RGB camera synchronized with the ECG. As a result, our proposed method of detecting pulse waves from the sole was observed to measure the peak interval of pulse waves with similar accuracy to an electrocardiogram.
Author(s): Georgia Harris, Iain Styles, Antonio Belli, Pola Goldberg Oppenheimer, University of Birmingham (United Kingdom)
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Raman Spectroscopy (RS) and machine learning are explored for the rapid, real-time, sensitive application of neurological diagnostics through the human eye. Biochemical information is obtained of fatty porcine tissue and flat-mounted porcine retinal samples using an in-house built, portable RS system. RS and FUNDUS imaging have been combined with a phantom eye model to obtain spectra under eye-safe parameters in an in-vivo environment, with analysis identifying carotenoid peaks of the macular pigment. This system has the potential to detect acute, biochemical changes indicative of neurodegenerative disorders such as Traumatic Brain Injury for early and accurate diagnoses, crucial for neurological recovery.
Author(s): Maksim Ryabkov, Elena B. Kiseleva, Privolzhsky Research Medical Univ (Russian Federation); Lev A. Matveev, Alexander A. Moiseev, Vladimir Yu Zaitsev, Institute of Applied Physics of the Russian Academy of Sciences (Russian Federation)
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Resection of sympathetic ganglia was performed in rabbits using the retroperitoneal approach. Lymph circulation in the small intestine wall was evaluated before and after the surgery using optical coherence tomography with lymphangiography (OCL) function. The signal attenuation coefficient was quantitatively analyzed, and the areas that did not scatter or absorb light in the infrared range were assigned to the lymph. Dysfunction of the intestinal lymphatic system in the acute period of spinal trauma was manifested by a decrease in total density of lymphatic vessels in all layers of the intestinal wall
Author(s): Brady Hunt, Samuel S. Streeter, Alberto J. Ruiz, Brian W. Pogue, Dartmouth College (United States)
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In vivo fluorescence imaging can provide quantitative image guidance in many diagnostic applications. However, most fluorescence measurement systems require bulky and/or expensive instrumentation, including external light sources, filtering components, and highly sensitive detectors. We have developed a simple clip-on attachment appropriate for contact-based fluorescence imaging using only the built-in light source and ultrawide camera sensor of a smartphone. The proposed system achieves a very compact form factor and can be readily fabricated using widely available low-cost materials. In vivo fluorescence quantification in murine and human skin post application of applying aminolevulinic acid gel using this system is demonstrated.
Author(s): Haimabati Dey, Peter Bermel, Purdue Univ (United States)
Author(s): Ilya Fine, Alexander Kaminsky, Elfi-Tech Ltd (Israel)
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This article discusses the origin of PPG signals. Two plausible hypotheses are analyzed: the volumetric hypothesis, and a model describing the signal in terms of the RBC aggregation. The theoretical approximations representing both models were elaborated. Experimentally, the PPG signals at the fingertip were measured while external pressure was applied to the fingertip. The experimental results agree with the theoretical predictions consistent with the aggregation model. In addition, the oscillometric signal and the PPG were simultaneously measured. The obtained results are not consistent with the volumetric hypothesis. All of the foregoing results support the argument favoring the proposed aggregation mechanism.
Conference Chair
Texas A&M Univ. (United States)
Program Committee
Zane A. Arp
U.S. Food and Drug Administration (United States)
Program Committee
Vanderbilt Univ. (United States)
Program Committee
The Univ. of Toledo (United States)
Program Committee
Univ. of Latvia (Latvia)
Program Committee
Fachhochschule Südwestfalen (Germany)
Program Committee
Texas A&M Univ. (United States)
Program Committee
Texas A&M Univ. (United States)
Program Committee
Swansea Univ. (United Kingdom)
Program Committee
Univ. of Arkansas (United States)
Program Committee
Univ. of California, Los Angeles (United States)
Program Committee
Florida International Univ. (United States)
Program Committee
International Collaboration On Repair Discoveries (Canada)