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Proceedings Paper

Improvement of in vivo rat skin optical clearing with chemical penetration enhancers
Author(s): Jing Wang; Xue Zhou; Shu Duan; Zhongwei Chen; Dan Zhu
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Paper Abstract

Optical method plays an important role in clinical diagnosis and treatment, but suffers from limited penetration depth of light in turbid tissue. The optical clearing technique can improve the light delivery significantly through immersion of tissues into Optical Clearing Agents (OCAs). However, the barrier function of stratum corneum makes it difficult for optical clearing of skin by topical application of OCAs. Addition of penetration enhancers to OCAs can improve the skin clearing efficacy, but most investigations were performed on in vitro skin. Here, to evaluate the efficacy of this method on in vivo skin, direct observation and measurement of diffuse reflectance spectra were performed after topical application of different mixtures. One OCA, PEG-400, and three penetration enhancers (PEs), Thiazone, Azone and Propylene Glycol (PG), were used. The results indicated that the addition of penetration enhancers could improve the optical clearing efficacy of rat skin in vivo significantly, the dermal blood vessels could be observed directly with PEs. Among the three penetration enhancers, Thiazone induced the largest enhancement of clearing efficacy, and the enhancement induced by PG is the least. This study is very helpful for in vivo application of OCAs to enhance skin optical clearing non- invasively.

Paper Details

Date Published: 17 February 2011
PDF: 7 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830Y (17 February 2011); doi: 10.1117/12.874859
Show Author Affiliations
Jing Wang, Huazhong Univ. of Science and Technology (China)
Xue Zhou, Huazhong Univ. of Science and Technology (China)
Shu Duan, Huazhong Univ. of Science and Technology (China)
Zhongwei Chen, Huazhong Univ. of Science and Technology (China)
Dan Zhu, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory M.D.; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg M.D.; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong M.D.; Justus F. R. Ilgner M.D.; Bodo E. Knudsen M.D.; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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