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Proceedings Paper

Measurement of human embryonic stem cell in the growing cycle
Author(s): X. Li; L. Zhao; Steve K. W. Oh; W. K. Chong; J. K. Ong; Allen K. Chen; Andre B. H. Choo
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Paper Abstract

A measurement and imaging system has been developed for in-line continuous measurement of live, unmodified, human embryonic stem cells (hESC). The measurement will not affect cell growth, structure, sterility and suitability for clinical use. The stem cell imaging system (SCIS) can be used to support the optimization of automated stem cell growth for invitro study and for high-volume bio-manufacture. This paper present the experimental and analysis for the optimization of system parameters. A non-linear lighting is developed to obtain a clear images. The individual cluster can be traced from day one to day two. The whole system is calibrated with measurement microscope and haemacytometer. The measurement accuracy is better than 90%.

Paper Details

Date Published: 3 October 2008
PDF: 8 pages
Proc. SPIE 7155, Ninth International Symposium on Laser Metrology, 715535 (3 October 2008); doi: 10.1117/12.814615
Show Author Affiliations
X. Li, Singapore Institute of Manufacturing Technology (Singapore)
L. Zhao, Singapore Institute of Manufacturing Technology (Singapore)
Steve K. W. Oh, Bioprocessing Technology Institute (Singapore)
W. K. Chong, Singapore Institute of Manufacturing Technology (Singapore)
J. K. Ong, Singapore Institute of Manufacturing Technology (Singapore)
Allen K. Chen, Bioprocessing Technology Institute (Singapore)
Andre B. H. Choo, Bioprocessing Technology Institute (Singapore)

Published in SPIE Proceedings Vol. 7155:
Ninth International Symposium on Laser Metrology
Chenggen Quan; Anand Asundi, Editor(s)

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