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Proceedings Paper

Aberration free refocusing for high numerical aperture microscopy
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Paper Abstract

In this paper, we present a novel technique that permits sectioning microscopes to refocus and acquire images from a large range of specimen depths without introducing movements near the specimen. In contrast to other such remote focusing methods, this technique avoids systematic aberrations that degrade image quality when imaging planes away from the optimal focal plane. Furthermore, the specific geometry that is employed in this work enables refocusing to be carried out at high speed and hence permits, for the first time, a number of dynamical biological processes to be observed. Although this technique can be applied to any optical imaging system, it is particularly suited to the case of high numerical aperture microscope systems. To demonstrate this we present results from two prototype systems built in our laboratory based on a slit scanning confocal fluorescence microscope and a two photon fluorescence microscope.

Paper Details

Date Published: 12 February 2008
PDF: 12 pages
Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686110 (12 February 2008); doi: 10.1117/12.771419
Show Author Affiliations
Edward Botcherby, Univ. of Oxford (United Kingdom)
Rimas Juškaitis, Univ. of Oxford (United Kingdom)
Martin Booth, Univ. of Oxford (United Kingdom)
Tony Wilson, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 6861:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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