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Proceedings Paper

Development of collinear transmission plasmonic biosensor for detection of HIV-1
Author(s): Saturnin Ombinda-Lemboumba; Sello Manoto; Charles Maphanga; Rudzani Malabi; Patience Mthunzi-Kufa
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Paper Abstract

Surface Plasmon Resonance (SPR) which is widely used to study interactions between different types of biomolecules, has emerged as a technique of choice for rapid and quantitative analyses. However, there are still some challenges on the use of the classical SPR optical configuration. The prism-based configuration setup requires precise alignment of light onto the sample surface and the oblique reflection angle plane yield optical aberration. In this work we have built, characterized and optimized a simple collinear transmission geometry plasmonic system for the detection of HIV-1. Here, a continuous wave laser at 785 nm with power output of 300 mW was used as light source and a 40X objective lens coupled to a CCD camera was used to collect and detect the transmitted intensity change. Furthermore, a white light source was used to study the wavelength dependency of the sample. We present our findings which may be useful to develop biomedical devices for point-of-care diagnostics and healthcare applications.

Paper Details

Date Published: 21 February 2020
PDF: 9 pages
Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570L (21 February 2020); doi: 10.1117/12.2546496
Show Author Affiliations
Saturnin Ombinda-Lemboumba, CSIR National Laser Ctr. (South Africa)
Sello Manoto, CSIR National Laser Ctr. (South Africa)
Charles Maphanga, CSIR National Laser Ctr. (South Africa)
Rudzani Malabi, CSIR National Laser Ctr. (South Africa)
Univ. Of South Africa (South Africa)
Patience Mthunzi-Kufa, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)
Univ. of Kwazulu Natal (South Africa)

Published in SPIE Proceedings Vol. 11257:
Plasmonics in Biology and Medicine XVII
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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