Surface Plasmon Resonance (SPR) which is widely used to study interactions between different types of biomolecules, has emerged as a technique of choice for rapid and quantitative analyses. However, there are still some challenges on the use of the classical SPR optical configuration. The prism-based configuration setup requires precise alignment of light onto the sample surface and the oblique reflection angle plane yield optical aberration. In this work we have built, characterized and optimized a simple collinear transmission geometry plasmonic system for the detection of HIV-1. Here, a continuous wave laser at 785 nm with power output of 300 mW was used as light source and a 40X objective lens coupled to a CCD camera was used to collect and detect the transmitted intensity change. Furthermore, a white light source was used to study the wavelength dependency of the sample. We present our findings which may be useful to develop biomedical devices for point-of-care diagnostics and healthcare applications.

Date Published: 21 February 2020
PDF: 9 pages
Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570L (21 February 2020); doi: 10.1117/12.2546496

Published in SPIE Proceedings Vol. 11257:
Plasmonics in Biology and Medicine XVII
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)