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Proceedings Paper

Mesoscale imaging of neuronal activity coupled with light-evoked motor mapping reveal movement-specific spatiotemporal patterns of cortical activation in awake mice
Author(s): Francesco Resta; Anna Letizia Allegra Mascaro; Elena Montagni; Giuseppe de Vito; Alessandro Scaglione; Francesco Saverio Pavone
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Paper Abstract

We developed an all-optical system that couples large-scale cortical imaging with chronic light-based motor mapping in awake mice. By AAV-mediated cortical transfection, we induced the co-expression of the red-shifted genetically encoded calcium indicator and a light-sensitive optogenetic actuator ChR2 over both the rostral and caudal forelimb areas, which was stable over several months. No evidence of cross-talk was detected during illumination of ChR2+ neurons with the light source used for RCaMP1a excitation. Light-based motor mapping coupled with wide-field imaging of neuronal activation in awake mice revealed spatiotemporal patterns of cortical activation specific for movement category.

Paper Details

Date Published: 21 February 2020
PDF: 4 pages
Proc. SPIE 11226, Neural Imaging and Sensing 2020, 112260I (21 February 2020); doi: 10.1117/12.2544214
Show Author Affiliations
Francesco Resta, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. degli Studi di Firenze (Italy)
Anna Letizia Allegra Mascaro, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Istituto di Neuroscienze, Consiglio Nazionale delle Ricerche (Italy)
Elena Montagni, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Giuseppe de Vito, Univ. degli Studi di Firenze (Italy)
Alessandro Scaglione, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. degli Studi di Firenze (Italy)
Francesco Saverio Pavone, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)


Published in SPIE Proceedings Vol. 11226:
Neural Imaging and Sensing 2020
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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