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Proceedings Paper

Adaptive optics two-photon microscopy for in vivo imaging of cortex and hippocampus in mouse brain
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Paper Abstract

Two-photon microscopy is a powerful tool for in vivo brain imaging that has greatly facilitated the neuroscience research in the past few decades. However, it still remains a challenge to image deep inside the brain with near diffraction-limited resolution due to the optical aberrations induced by the biological tissue and the cranial window. Here, we used an adaptive optics approach based on direct wavefront sensing to correct the aberration induced by the thinned skull window and achieved minimally invasive imaging of cerebral cortex with near-diffraction-limit resolution. Besides, by compensating the intrinsic aberration of a miniature gradient-index lens that implanted into the brain, two-photon imaging of hippocampal dendritic spines was realized over an extended field of view. The improvement in fluorescence intensity and imaging resolution enabled us to resolve the fine structures in live mouse brain such as dendritic spines that were invisible without the help of adaptive optics.

Paper Details

Date Published: 1 March 2019
PDF: 7 pages
Proc. SPIE 10865, Neural Imaging and Sensing 2019, 108650M (1 March 2019);
Show Author Affiliations
Congping Chen, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Zhongya Qin, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Sicong He, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Caleb Lui, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Nancy Y. Ip, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 10865:
Neural Imaging and Sensing 2019
Qingming Luo; Jun Ding; Ling Fu, Editor(s)

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