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Proceedings Paper

A fiber-laser-based stimulated Raman scattering spectral microscope
Author(s): Keisuke Nose; Yasuyuki Ozeki; Tatsuya Kishi; Kazuhiko Sumimura; Yasuo Kanematsu; Kazuyoshi Itoh
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Paper Abstract

Stimulated Raman scattering (SRS) spectral microscopy is a powerful technique for label-free biological imaging because it allows us to distinguish chemical species with overlapping Raman bands. Here we present an SRS spectral microscope based only on fiber lasers (FL’s), which offer the possibilities of downsizing and simplification of the system. A femtosecond figure-8 Er-FL at a repetition rate of 54.4 MHz is used to generate pump pulses. After amplified by an Er doped fiber amplifier, Er-FL pulses are spectrally compressed to 2-ps second harmonic pulses. For generating Stokes pulses, a femtosecond Yb-FL pulses at a repetition rate of 27.2 MHz is used. Then these lasers are synchronized by a phase locked loop, which consists of a two-photon absorption photodetector, a loop filter, a phase modulator in the Er- FL cavity, and a piezo electric transducer in the Yb-FL cavity. The intensity noise of pump pulses is reduced by the collinear balanced detection (CBD) technique based on delay-and-add fiber lines. Experimentally, we confirmed that the intensity noise level of probe pulses was close to the shot noise limit. The Stokes pulses are introduced to a wavelength tunable band pass filter (BPF), which consists of a galvanomirror scanner, a 4-f optical system, a reflection grating, and a collimator. This system is able to scan the wavenumber from 2850 cm-1 to 3100 cm-1 by tuning the BPF. We succeeded in the spectral imaging of a mixture of polystyrene beads and poly(methyl methacrylate) beads.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880Y (22 February 2013); doi: 10.1117/12.2004275
Show Author Affiliations
Keisuke Nose, Osaka Univ. (Japan)
Yasuyuki Ozeki, Osaka Univ. (Japan)
PRESTO, Japan Science and Technology Agency (Japan)
Tatsuya Kishi, Osaka Univ. (Japan)
Kazuhiko Sumimura, Osaka Univ. (Japan)
Yasuo Kanematsu, Osaka Univ. (Japan)
Kazuyoshi Itoh, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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