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Proceedings Paper

Laser-induced autofluorescence of cells and tissue
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Paper Abstract

The laser induced in-vivo autofluorescence in the visible spectral region of cells and tissues was studied using stationary and time-resolved fluorescence measurements as well as video- intensified- and confocal laser-scanning microscopy. Due to their excitation and emission spectra and their decay kinetics (ps- and ns-region) at least three intrinsic fluorophores were so far differentiated and attributed to the reduced pyridine coenzymes NADH/NADPH, free and bound flavin molecules and endogenous porphyrins. It was found, that defects in the mitochondrial respiratory chain as well as photodynamically or chemically induced cell destruction results in an increase of the fluorescence intensity in the blue/green spectral range.

Paper Details

Date Published: 27 August 1993
PDF: 9 pages
Proc. SPIE 1887, Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods, (27 August 1993); doi: 10.1117/12.151184
Show Author Affiliations
Karsten Koenig, Univ. Ulm (Germany)
Herbert Schneckenburger, Univ. Ulm (Germany)
Angelika C. Rueck, Univ. Ulm (Germany)
Rudolf W. Steiner, Univ. Ulm (Germany)
Heinrich Walt, University Hospital (Switzerland)

Published in SPIE Proceedings Vol. 1887:
Physiological Imaging, Spectroscopy, and Early-Detection Diagnostic Methods
Randall Locke Barbour; Mark J. Carvlin, Editor(s)

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