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Submissions to this conference may include the following to improve the chance for acceptance:
  • 100-word text abstract (for online program) (REQUIRED)
  • 250-word text abstract (for abstract digest) (REQUIRED)
  • 2-page extended abstract (for committee review only) (OPTIONAL). In order to be nominated for the award, an extended abstract must be submitted. We are expecting to get 2-page extended abstract for committee review only. The extended abstract must be submitted as a separate document limited to two pages, including tables and figures.
All submissions will be peer-reviewed by the Program Committee. Include author names and affiliations; text; any figures, tables, or images; and sufficient data to permit committee review. Extended abstracts will be used only for the purpose of peer review for Prizmatix young investigator awards, and will not be published.

Novel applications and/or solutions to technological problems involving i) the use of nanostructures, nanoparticles, metamaterials and nanostructured materials for biological applications; ii) photonic studies of nanoscale interactions in biology and medicine; and iii) the use of biological materials/templates for the development of nanostructured nanophotonic imaging and sensing devices are significant foci of both industrial and academic research. These applications and solutions are inherently interdisciplinary by nature and thus require a seamless transfer of knowledge between physics, chemistry, biology, medicine and engineering.

Recent integrative research efforts have included, for example, (a) nanotechnology (fabrication and application) as a tool in developing new, and improving existing, optical imaging techniques for real-time sub-wavelength imaging of cellular processes, (b) developing the next generation of nano-biosensors for improving biological / chemical sensing applications, (c) using nanoparticles / nanostructures for optical engineering of methodologies for targeting and treatment of disease, (d) applying computer and information technologies in the development of new models and data analysis for understanding cellular mechanisms, (e) developing new photonic devices and systems that are hybrids of traditional polymeric and semiconductor materials with biological materials; (f) very large scale and/or very sensitive detection down to single molecule level for drug discovery and diagnostics applications, such as nanoarrays; and (g) quantum computing and machine learning to biomedical applications.

The objective of this conference is to bring together scientists and researchers interested in the latest advances in the advancement of materials and methods that combine nanophotonics with biology. More specifically, this conference is to discuss the development of processing, characterization, and simulation of bioinspired and bioderived nanophotonic structures, metamaterials for sub-wavelength imaging, nanoscale interactions in biological systems, functionalized nanoparticles for biological applications and the use of nanostructures / nanoparticles for high throughput analysis (nanoarrays).

The conference will focus on four streams of contributions:
Papers from industry, government, academia, and other research organizations are solicited on the following and related topics:
Prizmatix Young Investigator Awards
Three "Young Investigator Awards", 500 US$ each, sponsored by Prizmatix Ltd., will be awarded for notable contributions by young scientists presenting their work at the Conference (talk or poster). In order to be nominated for the award an extended abstract must be submitted.

We are expecting to get 2-page extended abstract for committee review only. The extended abstract must be submitted as a separate document limited to two pages, including tables and figures.
All submissions will be peer-reviewed by the Program Committee. Extended abstracts will be used only for the purpose of peer review for Prizmatix Young Investigator Awards, and will not be published.
In progress – view active session
Conference 11976

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX

In person: 24 January 2022
View Session ∨
  • 1: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications I
  • 2: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications II
  • 3: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications III
  • 4: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications IV
  • Posters
  • Nano/Biophotonics Plenary Session


  • Submissions are accepted through 06-December
  • Notification of acceptance by 20-December

View Call for Papers PDF Flyer
Session 1: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications I
Session Chair: Dror Fixler, Bar-Ilan Univ. (Israel)
Author(s): Christian Oelsner, Uwe Ortmann, Eugeny Ermilov, Volker Buschmann, Matthias Patting, Rainer Erdmann, PicoQuant (Germany)
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Up-conversion nanoparticles are highly attractive for application cases in bio sensing and imaging without autofluorescence. Characterizing the photophyiscial properties of such nanoparticles is essential to enhance the efficiency of preparation methods as well as their electronic and optical properties. We will demonstrate the performance of a spectrometer-microscope assembly for characterization and analysis of up-conversion nanoparticles in terms of lifetime, spectral, and spatial resolution, which provides more information than when using only lifetime or steady-state experiments.
Author(s): Jhon James Hernández Sarria, Universidade de São Paulo (Brazil)
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The use of enhanced electromagnetic fields in nanophotonic structures is moving from plasmonic to all-dielectric platforms in order to overcome the intrinsic Ohmic losses from metal inclusions. In this work, we demonstrate that strong electromagnetic fields may be generated in the infrared without heating the nearby molecules by exciting anapole modes in a slotted all-dielectric cylinder on a glass substrate surrounded by water. Numerical calculations are used to prove that the platform is useful to manipulate small biomolecules without heating.
Author(s): Hajun Yoo, Hongki Lee, Gwiyeong Moon, Changhun Lee, School of Electrical and Electronic Engineering, Yonsei University (Korea, Republic of); Jeon-Soo Shin, Yonsei University College of Medicine (Korea, Republic of); Donghyun Kim, School of Electrical and Electronic Engineering, Yonsei University (Korea, Republic of)
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In this work, we have investigated super-resolution nanospeckle illumination microscopy using randomly localized near-field speckle by disordered nanocomposite islands. Nanoscale island patterns can be mass-produced without any lithography on the substrates, effectively generating randomly localized plasmonic nanospeckle. A modified blind structured illumination microscopy algorithm was applied with azimuthal scanning illumination satisfying the constraint of the approximate homogeneity of the nanospeckle pattern. Through an experimental study using 100-nm fluorescent nanobeads and the distribution of gangliosides in the HeLa cell membrane, it was confirmed that the spatial resolution was improved more than 3-times compared to the diffraction-limited system.
Author(s): Moti Fridman, Bar-Ilan Univ (Israel)
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We experimentally demonstrate ghost imaging in the frequency domain based on a speckle pattern as a reference. Our scheme can measure spectrum with high resolution. We perform the spectral measurement by a time stretch system that maps from frequency to time. We achieve a reconstruction of our signal by analyzing the output spectrum while shifting the speckle field.
Author(s): Channa Shapira, Dror Fixler, Hamootal Duadi, Inbar Yariv, Bar Ilan Univ (Israel)
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Imaging inside a turbid media is range limited, while sensing the medium is possible in larger depths using the iterative multi-plane optical properties extraction technique. It analyzes the reemitted light phase image reconstructed from the iterative multi-plane Gerchberg-Saxton algorithm. The root mean square of the phase yields two graphs with opposite behaviors, that cross each other in 〖μ'〗_(s,cp). The graphs enable the extraction of the reduced scattering coefficient, 〖μ'〗_s. In this work, we aim to extend the range of 〖μ'〗_s detection by optical magnification. We use a modified diffusion theory and show 〖μ'〗_(s,cp) shifts with the magnification. The theoretical results were then tested experimentally, using phantoms with varying scatterings coefficients.
Author(s): Maksim O. Makarenko, Arturo Burguete-Lopez, Fedor Getman, Andrea Fratalocchi, King Abdullah Univ of Science and Technology (Saudi Arabia)
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This work presents an AI-driven framework to extract the biological tissue's refractive index and thickness maps from a single RGB image. This approach is based on a physical light-trapping surface and an unsupervised inverse search projector which projects given RGB pixel to the sample's refractive index and thickness at the corresponding coordinate.
Session 2: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications II
Session Chair: Euan McLeod, Wyant College of Optical Sciences (United States)
Author(s): Rinat Ankri, Ariel University (Israel)
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Current methods for assessing the immune status of a patient and tumor are limited, which has led to an increase interest in the development of reliable, noninvasive, real-time means to image and trace small groups of cells within large tissue volumes and continuously evaluate their biodistribution, final destination, and functionality post- transplantation. We will present an optical-based near infrared (NIR) fluorescence lifetime (FLT) imaging method and custom-made contrast agents for cells tracking imaging. We will show phasor FLT analysis of immune cells tracking under tissue conditions, paving the way for further investigation of immune cells activation state at tumor site.
Author(s): Donato Conteduca, Thomas F. Krauss, Univ of York (United Kingdom)
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Extracellular vesicles (EVs) have recently been recognized as a promising marker for the early detection of cancer. Many technologies are used to isolate and analyze EVs, such as optical and electrochemical sensors, however a technique that can achieve high sensitivity together with a multiplexing capability remains a challenge. Here, we demonstrate the ability to detect very low concentrations of vesicles using a dielectric metasurface approach, verifying a resolution <10^3 EV/mL and high selectivity with vesicles surface-functionalised with CD9 proteins, a typical cancer biomarker, also integrating multiple sensors on the same platform. Our sensors offers a path towards personalised cancer medicine.
Session 3: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications III
Session Chair: Euan McLeod, Wyant College of Optical Sciences (United States)
Author(s): Cheng Li, Sartanee Suebka, Wyant College of Optical Sciences (United States); Judith Su, Wyant College of Optical Sciences (United States), Department of Biomedical Engineering (United States)
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Thermal effects need to be accurately measured and/or controlled to generate continuous kinetic binding curves with whispering gallery mode (WGM) microcavity sensors. We use a high spatial resolution optical frequency domain reflectometry system at 780 nm to capture the Rayleigh backscattering signal within a microtoroid optical resonator for temperature calibration. It is shown that this system has a temperature detection accuracy of 30 mK. This technique characterizes thermal effects in the microcavity and the surrounding environment, thus enabling lower limits of detection to be achieved.
Author(s): Conrad Corbella Bagot, Eric Rappeport, Ananda Das, Wounjhang Park, Univ of Colorado Boulder (United States)
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Many schemes have been proposed to measure physiological pH by conjugating pH-sensitive dyes with Upconverting Nanoparticles (UCNPs). However, the signal transduction is typically achieved by a combination of photon reabsorption and Förster resonant energy transfer (FRET) between UCNPs and dyes. While FRET senses the pH in the immediate vicinity of the sensor, photon reabsorption is strongly affected by the global environment, potentially obscuring the local pH values. In this presentation, we report a new sensing scheme that detects only the contributions by FRET and is insensitive to photon reabsorption, making it the first demonstration of truly local pH measurements.
Author(s): Xianglong Miao, Peter Q. Liu, University at Buffalo (United States)
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We demonstrate a proof-of-concept design of a new type of nanophotonic molecular sensors with the functionality of trapping target biomolecules to the device regions of highly enhanced field intensity, and hence facilitating improvement of sensing performance. Our experimental results show that these devices are capable of effectively accumulating precipitated L-Proline after the analyte solution of various concentrations dries on the device surface. Specifically, our devices produce a few percentage reflection (absorption) change in response to picogram level amino acid proline. Our work demonstrates a new strategy for designing optical sensors for detecting and sensing trace amount of analyte such as molecules in solutions.
Author(s): Meruyert Imanbekova, McGill Univ (Canada); Tatu Rojalin, Randy Carney, John Voss, University of California Davis (United States); Sebastian Wachsmann-Hogiu, McGill Univ (Canada)
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The presented study describes the specific Raman spectroscopic fingerprint of the Aβ protein that is associated with small extracellular vesicles. The results revealed secondary structure and the size of the Aβ enclosed in sEVs, and its effect on the sEVs plasma membrane. Our findings support the application of Raman spectroscopy for the detection and characterization of sEVs carrying potential biomarkers of neurodegenerative diseases.
Author(s): Juanjuan Liu, McGill Univ (Canada); Fartash Vasefi, SafetySpect Inc. (United States); Sebastian Wachsmann-Hogiu, McGill Univ (Canada)
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We developed a nanocomposite substrate that is composed of metallic nanoparticles deposited on a commercial glucose test strip, which can improve specificity and sensitivity via SERS and EC. This sensing substrate is utilized for chlorfenapyr detection, where it is functioning as a working electrode for EC and a SERS active substrate for Raman measurements. Compared to the traditional techniques used for chlorfenapyr detection, this multimodal sensor is inexpensive and has a fast response time. It shows potential for point-of-need applications for food safety.
Session 4: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications IV
Session Chair: Sebastian Wachsmann-Hogiu, McGill Univ. (Canada)
Author(s): Euan McLeod, University of Arizona (United States)
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Current 3D fabrication approaches either do not have nanoscale resolution, or are severely limited in terms of materials or structure. Here we present optical positioning and linking (OPAL), which uses optical tweezers and biotin-avidin linkage chemistry to assemble previously infeasible metamaterial and biosensor structures out of building blocks. These building blocks include polystyrene microspheres, gold nanospheres, and silica-gold nanoshells. We present the precision assembly of >440 particles into a 3D structure, as well as the fastest published optical tweezer nanoparticle manipulation speeds. Structures are designed using the coupled dipole method, which is particularly fast for these types of structures.
Author(s): Isabel Barth, Thomas F. Krauss, University of York (United Kingdom)
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We previously demonstrated how the combination of common-path interferometry and dielectric nanostructures considerably improves the limit of detection of label-free biochemical sensing and enables the challenging detection of biomarkers for infection at clinically relevant concentrations of picograms per milliliter. We are now developing this technology as a system without imaging lenses in a further drive towards miniaturization and simplicity for low-cost point-of-care diagnostics by altering the purpose of the resonant nanostructure: in addition to its function as core sensing element, the nanostructures are now designed as diffractive element. The result will be a self-referencing common-path interferometric system with multiplexing capabilities.
Author(s): Reza Abbasi, Juanjuan Liu, Sebastian Wachsmann-Hogiu, McGill University (Canada)
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In ECL biosensors, a sophisticated instrument is commonly employed to monitor emitted light from the chemiluminescence reaction. Therefore, these biosensors may not be practical for point-of-care (POC) testing that is significantly important in healthcare diagnostics. In this work, microfluidic and luminol-based-ECL systems were integrated on a CMOS chip to build a miniaturized ECL sensor for POC applications. The results showed that this novel lab-on-chip system can detect uric acid levels as an important biomarker for the diagnosis of gout disease in urine and saliva lower than the physiological range. The repeatability, reproducibility and selectivity of the device were also studied.
Author(s): Maryam Baker, Weilin Liu, Euan McLeod, Wyant College of Optical Sciences (United States)
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Scattering from nanoparticles has previously been utilized to achieve sub-wavelength resolution in microscopy, sub-diffraction beam widths in focused laser beams, and improved sensitivity in biological sensing. However, these applications often require time-consuming detailed vectorial simulation of the interaction of incident fields with the nanoparticles to achieve the desired performance. On the other hand, the scalar angular spectrum method is widely used for rapid holographic reconstruction, but can be inaccurate for sub-wavelength features, depending on the light-matter interaction model. Here we establish the domains of accuracy of three scalar light-matter interaction models for arrays of randomly distributed dielectric and metallic nanoparticles.
Author(s): Xintao Zhao, Somin Eunice Lee, Univ of Michigan (United States)
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Cell migration is an essential cellular process that help cells to develop complex organisms, organs, and tissues, arrange cells into specialized architecture and help organize the nervous system. Nanoscale imaging has the potential to provide new insight into the mechanics of cell migration. However, quantification over time in nanoscale imaging remains difficult. Here, we visualize cell migration by non-bleaching nanoscale imaging. We present a set of quantitative metrics - length, branching, gaps, distribution, and curvature - to quantitatively analyze cell migration at the nanoscale over time.
Author(s): Feng Liang, Anesthesia department of Mass General Hospital (United States)
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Perioperative neurocognitive disorder (PND), including postoperative delirium, delayed neurocognitive recovery, and postoperative neurocognitive disorder can increase morbidity, mortality, and the cost of medical care. However, the pathogenesis and biomarker of PND remain largely to be determined. The proposed research will use a newly developed and innovative Nanoneedle and Nanobeam technology to set up a system to establish blood (or urine, feces and saliva) Tau and phosphorylated Tau levels as the biomarker(s) of PND as well as single molecular level study the Neuropathogenesis .
Conference attendees are invited to attend the BiOS poster session. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field. :: :: View poster presentation guidelines and set-up instructions at: :: [{}+{}]
Author(s): Sandhya Clement, The University of Sydney (Australia); Anna Guller, Macquarie University (Australia); Saabah B. Mahbub, Ewa M. Goldys, Univ of New South Wales (Australia)
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Photodynamic therapy (PDT) is an anti-cancer treatment method, non-invasive and relatively safe, which utilizes a photosensitiser drug and a light source to treat the superficial lesions. This conventional PDT is not applicable to the deep-seated tumours, due to the limited tissue penetration of the light used for the therapy. Herein, we proposed an efficient modification to PDT, the radiodynamic therapy (RDT), in which light source is replaced by X-ray to activate a nanoformulated drug to generate anti-cancer cytotoxic effect in pancreatic cancer cells in conventional and hypoxic in vitro cell cultures and in a 3D tissue engineered metastatic tumour model.
Author(s): Shweta V. Pawar, Bar ilan university (Israel)
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In today’s research area it is extremely important to assemble nanomaterials into electric devices at the nanoscale level due to the rapid expansion of nanotechnology in various fields. Designing a nanohybrid composed of gold nanoparticles (AuNPs) and red-emitting carbon dots (CDs) can be used to develop a fluorescence lifetime imaging (FLIM) based logic gate that can respond to multiple input parameters .The AuNPs are conjugated to CDs surfaces through a strong covalent linkage between them. These fluorescence lifetimes-based logic gates could be the new way to overcome the limitation of fluorescence intensity-based logic gates. The Au-CDs nanohybrid shows significant fluorescence quenching of pristine CDs after conjugation of AuNPs. This quenched fluorescence signal can be recovered back by using a proper recovering agent giving us a reversible logic output. This nanohybrid device can be used to construct complex logic functions that are independent of concentrations.
Author(s): Sung Jin Kim, University of Miami (United States); Ju-Hyung Yun, Incheon National University (Korea, Republic of)
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The hybrid metal/quantum dots (QDs) structures were synthesized and characterized with different thicknesses of silica shell by uncomplicated and typical methods. By engineering the shell thickness and Au nanoparticles (Au NPs) core size with shapes, the interaction between Au NPs and QDs could be adjusted flexibly so that the energy transfer process could be witnessed thoroughly. The improvement of the emission intensity and the reduction in the PL lifetime with the appropriate thickness of silica layer were obtained. Likewise, the energy transfer efficiency between QDs donor – acceptor pair was inferred as the dominance of larger QDs with the existence of hybrid structures. The quenching of QDs donors and the enhancement of QDs acceptors in PL emission intensity were determined by the different core shell structure. Finally, as-prepared metal/silica/QDs structures have been explored to bio-imaging applications, which leading to enhanced light absorption and sensitivity.
Author(s): Pavitra S. Rudraiah, Hamootal Duadi, Dror Fixler, Bar Ilan university (Israel)
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Optical imaging and spectroscopy techniques study tissue optical properties. Here, we developed a fiber-based diffuse reflectance (DR) technique to achieve deeper tissue information. In our two-layer (2L) model, the thickness of the top layer was kept with a constant absorption (µa), thickness, and reduced scattering coefficients, while the µa of the bottom layers were varied. We found a unique crossover point (Cp) in the DR profile for the 2L structure and we extracted the slope before and after the Cp. The calculated absorption coefficients before the Cp are in good agreement with the absorption coefficients of the bottom layer.
Author(s): Inbar Yariv, Dror Fixler, Hamootal Duadi, Bar Ilan Univ (Israel)
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Deep tissue imaging using visible light is challenging due to its turbid nature. Nevertheless, clinical information can be detected by sensing changes in the tissue’s optical properties. This paper presents the multi-layer study of the reflection-based iterative multiplane optical property extraction (IMOPE) technique. Based on the reemitted light phase, the IMOPE detects the medium scattering properties, which are used as indicators of the internal tissue information. This work presents the study of single- and two-layer phantoms and a new phase image analysis that provides detection of different scattering layers. The IMOPE was applied for an iron-based nanoparticles drug detection in mice leishmaniasis lesions at different stages of the disease.
Author(s): Chen Tzror-Azankot, Oshra Betzer, Tamar Sadan, Mecachen Motiei, Rachela Popovtzer, Faculty of Engineering and the Institute of Nanotechnology & Advanced Materials, Bar-Ilan University (Israel)
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Tumor cells are characterized by high glucose demand, because of their increased requirement for energy. Taking advantage of this feature, we developed a glucose-labeled liposome, which is tumor-targeted by recognizing over-expressed glucose transporters on tumor cells. In this study, uptake of glucose-coated and non-coated liposomes was evaluated in different types of cancer cells. We found that glucose-coated liposomes were preferentially uptaken by cancer cells, mediated via glucose transporter-1. Moreover, cell lines with high metabolic activity exhibited higher uptake of glucose-coated liposomes, as compared to that of low metabolic activity cancer cells and non-cancerous cell lines.
Author(s): Mark A. Ciappesoni, Sung Jin Kim, University of Miami (United States)
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Optical Coherence Tomography (OCT) is a noncontact imaging technique that uses coherent light to capture two- and three- dimensional images from within scattering media with micrometer-resolution. A key benefit of OCT is its ability to take subsurface images of tissue, where axial resolution is limited by the bandwidth of the light source. We propose an OCT system using a large portion of the NIR spectrum (850-1350nm) using the plasmon enhanced field effect transistor (FET) allowing integrated detection. The plasmon FET detectors can span a broadband spectrum and efficiently convert and amplify incident light directly into electrical signals through coupling with plasmonic modes. These tailored plasmonic modes allow for a fully tunable detector spectral response from visible to NIR.
Author(s): Jacqueline Labovitz, Menachem Motiei, Tamar Sadan, Rachela Popovtzer, Bar-Ilan University (Israel)
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Nanoparticle-based gene therapy is a promising approach with enormous potential for treatment of various diseases. However, a major obstacle that prevents its clinical application is endosomal entrapment and subsequent degradation of the nanoparticle-gene therapy complex. Escape from the endosome by means of membrane disruption has been shown to greatly enhance the efficacy of gene therapy. This research explores a novel, multi-method approach, utilizing fluorescent confocal microscopy, as well as sophisticated image analysis, to provide a quantitative and uniform method that can denote the probability of specific nanoparticles in performing endosomal escape.
Author(s): Adi Anaki, Menachem Motiei, Tamar Sadan, Rachela Popovtzer, Bar-Ilan University (Israel)
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Exosomes are promising vectors for anti-tumor therapy due to their innate biological abilities. However, promoting clinical application of exosome-based therapeutics requires elucidation of key issues, including the ability to overcome biological barriers. Here, using gold nanoparticles (GNPs), we examined crucial parameters for drug delivery in exosomes derived from mesenchymal stem cells. This novel technology can promote clinical translation by providing fundamental knowledge on exosomes as therapeutic carriers for enhanced targeted drug delivery to tumors.
Nano/Biophotonics Plenary Session
In person: 25 January 2022 • 10:30 AM - 11:30 AM
Join us for the Nano/Biophotonics Plenary Session. This year's talk will be given by Hongjie Dai of Stanford Univ. (United States).
Conference Chair
Bar-Ilan Univ. (Israel)
Conference Chair
Ewa M. Goldys
The Univ. of New South Wales (Australia)
Conference Chair
McGill Univ. (Canada)
Program Committee
The Univ. of North Carolina at Charlotte (United States)
Program Committee
Lorena Betancor
Univ. ORT Uruguay (Uruguay)
Program Committee
Columbia Univ. (United States)
Program Committee
Gdansk Univ. of Technology (Poland)
Program Committee
Massachusetts General Hospital (United States)
Program Committee
Univ. of Miami (United States)
Program Committee
Dublin City Univ. (Ireland)
Program Committee
International Laser Ctr. (Slovakia)
Program Committee
Univ. at Buffalo (United States)