Simulations of imperfect refractive index matching in scanning laser optical tomography and a method for correction

Ole Hill; Merve Wollweber; Roland Lachmayer; Tammo Ripken

doi:10.1117/12.2649936

16 May 2023 Simulations of imperfect refractive index matching in scanning laser optical tomography and a method for correction

Ole Hill, Merve Wollweber, Roland Lachmayer, Tammo Ripken

Author Affiliations +

Proceedings Volume 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; 1238509 (2023) https://doi.org/10.1117/12.2649936
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Since additive manufacturing has become increasingly popular in prototyping, printed optics are also beginning to enter the market. Novel characterization methods for printed optics are needed because traditional, destructive methods often do not work on these optics. The scope of investigation is also different for additively manufactured optics. Homogeneity of subtractive manufactured optics such as glass lenses is usually granted but for printed optics the interfaces in-between layers can cause absorption, scattering or refraction. Functionalized optics can also have characteristics such as fluorescence that cannot be tested with traditional methods. The presented work tries to fill the void for this particular challenge by studying two non-destructive methods for optical characterization of such components and expanding their use by clever combination. In Scanning Laser Optical Tomography (SLOT), a needle-like beam is formed and focused into the sample. The sample is scanned to form projection images and rotated to allow for reconstruction, which yields volumetric data about scattering, transmission and fluorescence of sample structures. Simulated SLOT measurements with imperfect Refractive Index (RI) matching of sample and medium are presented. A method to correct distorted measurements is presented and evaluated. The simulations imply that a measurement with a RI mismatch of up to 0.1 can still yield reasonable results.

Conference Presentation

Citation Download Citation

Ole Hill, Merve Wollweber, Roland Lachmayer, and Tammo Ripken "Simulations of imperfect refractive index matching in scanning laser optical tomography and a method for correction", Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, 1238509 (16 May 2023); https://doi.org/10.1117/12.2649936

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