Review of life science applications using submicron O-PTIR and simultaneous Raman microscopy: a new paradigm in vibrational spectroscopy

Mustafa Kansiz; Alice Spadea; Jayakrupakar Nallala; Cassio Lima; Howbeer Muhamadali; Joanna Denbigh; Jayne Lawrence; Gorkem Bakir; Peter Gardner; Nick Stone; Roy Goodacre; Kathleen Gough; Oxana Klementieva

doi:10.1117/12.2578662

5 March 2021 Review of life science applications using submicron O-PTIR and simultaneous Raman microscopy: a new paradigm in vibrational spectroscopy

Mustafa Kansiz, Alice Spadea, Jayakrupakar Nallala, Cassio Lima, Howbeer Muhamadali, Joanna Denbigh, Jayne Lawrence, Gorkem Bakir, Peter Gardner, Nick Stone, Roy Goodacre, Kathleen Gough, Oxana Klementieva

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Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 1165617 (2021) https://doi.org/10.1117/12.2578662
Event: SPIE BiOS, 2021, Online Only

Abstract

The recent advent of Optical Photothermal IR (O-PTIR), has enabled for the first time, submicron infrared microscopy in far-field reflection mode with the combination of Raman for simultaneous, correlative IR+Raman microscopy. These unique and exciting synergistic capabilities are now spawning interest in life science application [1-2]. A broad range of life science applications, otherwise impossible with traditional FTIR/QCL microscopy, will be presented, ranging from live cell imaging in water, to ultra-high resolution images of breast tissue calcifications, amyloid aggregates in neurons (neurites and dendritic spines), individual collagen fibrils with polarized IR and individual isotopically labelled bacterial cells and more.

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Mustafa Kansiz, Alice Spadea, Jayakrupakar Nallala, Cassio Lima, Howbeer Muhamadali, Joanna Denbigh, Jayne Lawrence, Gorkem Bakir, Peter Gardner, Nick Stone, Roy Goodacre, Kathleen Gough, and Oxana Klementieva "Review of life science applications using submicron O-PTIR and simultaneous Raman microscopy: a new paradigm in vibrational spectroscopy", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 1165617 (5 March 2021); https://doi.org/10.1117/12.2578662

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