Power efficient, uniform TIRF illumination via a photonic lantern for quantitative super-resolution imaging
Total internal reflection fluorescence (TIRF) is a popular type of illumination used in fluorescence microscopy for surface imaging of biospecimens; however, the commonly used objective-based TIRF illumination can induce artifacts that degrade the image. We previously demonstrated a fiber bundle that reshaped an input beam to achieve artifact-free TIRF illumination with coherent or incoherent light sources. Here we demonstrate an improved design using a photonic lantern, which will increase the power throughput by reducing coupling losses at the input facet. The improved power efficiency will facilitate new capabilities such as super-resolution imaging.
Univ. of Central Florida (United States)
Ben Croop received his BSE in Materials Science and Engineering at the University of Michigan, his MSE in Materials Science and Engineering at the University of Washington, and his PhD in Optics and Photonics from the University of Central Florida. His work within fluorescence microscopy focuses on quantitative, intensity-based measurements from samples. These methods are used to assess the stoichiometry of protein complexes and produce uniform, artifact-free images of cell and tissue samples. Today he will present work on an improved, fiber-based beam shaping method for surface imaging of biological samples.