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Conference 11960 > Paper 11960-145
Paper 11960-145

OCT-guided contour scanning for dynamically-focused photoacoustic remote sensing microscopy

Abstract

Ultraviolet photoacoustic remote sensing (UV-PARS) microscopy is a non-contact imaging modality capable of producing label-free absorption contrast images of cell nuclei. This virtual hematoxylin-like imaging combined with virtual eosin-like data from 1310 nm scattering microscopy can provide complete virtual H&E histologically in unstained tissues. Here, we develop contour scanning for applying UV-PARS and scattering-based virtual histology in fresh and formalin-fixed thick tissues. Our spectral-domain OCT-guided approach initially scans specimens in 3D, and a custom algorithm extracts the surface contour. A high-resolution UV-PARS scan is then performed using a z-axis stage for dynamic focusing to compensate for sample surface irregularities.

Presenter

Univ. of Alberta (Canada)
Presenter/Author
Univ. of Alberta (Canada)
Author
Univ. of Alberta (Canada)
Author
Univ. of Alberta (Canada)
Author
Univ. of Alberta (Canada)
Author
Univ. of Alberta (Canada)
Author
Univ. of Alberta (Canada)