Presentation
5 March 2021 Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume
Author Affiliations +
Abstract
We will demonstrate the performance of a spectrometer-microscope assembly for characterization and analysis of samples in terms of lifetime, spectral and spatial resolution. This combined approach provides access to further information, which are not available when using only lifetime or steady-state experiments. The combination of both techniques in one setup can help to understand biochemical or physical processes by detecting changes in local environment such as pH, temperature, or ion concentration, and to identify molecular interactions or conformation changes via Förster Resonance Energy Transfer (FRET).
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Christian Oelsner, Eugeny Ermilov, Volker Buschmann, Frank Birke, Matthias Patting, Uwe Ortmann, and Rainer Erdmann "Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560H (5 March 2021); https://doi.org/10.1117/12.2576400
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KEYWORDS
Luminescence

Spectroscopy

Molecular spectroscopy

Molecules

Statistical analysis

Proteins

Resonance energy transfer

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