Imaging the structure and function of live mouse brain with multimodal nonlinear optical microscopy

Dan Fu

doi:10.1117/12.2577891

5 March 2021 Imaging the structure and function of live mouse brain with multimodal nonlinear optical microscopy

Dan Fu

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116561D (2021) https://doi.org/10.1117/12.2577891
Event: SPIE BiOS, 2021, Online Only

Abstract

The brain is an enormously complex organ that consumes a substantial amount of body energy. Understanding how brain function requires the ability to not only map out the cellular structure but also probe functional processes. Multiphoton fluorescence microscopy has played a crucial role in current investigations due to the wide variety of synthetic dyes and proteins available for imaging brain cells and neuronal activities at high spatial and temporal resolution. However, it has limited capability to resolve multiple features simultaneously. We combine multiphoton fluorescence with label-free nonlinear imaging techniques including transient absorption microscopy and stimulated Raman scattering microscopy to expand the structural and functional features that can be imaged simultaneously. With this platform, we demonstrate the reconstruction of axonal features and microvascular networks together with the mapping of cellular organization.

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Dan Fu "Imaging the structure and function of live mouse brain with multimodal nonlinear optical microscopy", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116561D (5 March 2021); https://doi.org/10.1117/12.2577891

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KEYWORDS

Brain

Microscopy

Neuroimaging

Optical microscopy

Axons

In vivo imaging

Luminescence

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