Best versatility/localization precision combination in single molecule localization microscopy enabled by adaptive optics: MicAO 3DSR

Fabrice Harms; Audrius Jasaitis; Cynthia Veilly; Xavier Levecq

doi:10.1117/12.2583292

5 March 2021 Best versatility/localization precision combination in single molecule localization microscopy enabled by adaptive optics: MicAO 3DSR

Fabrice Harms, Audrius Jasaitis, Cynthia Veilly, Xavier Levecq

Author Affiliations +

Proceedings Volume 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV; 116500G (2021) https://doi.org/10.1117/12.2583292
Event: SPIE BiOS, 2021, Online Only

Abstract

PSF engineering has been widely used over the past years to enable 3D localization in Single Molecule Localization Microscopy (SMLM) techniques, such as PALM/STORM superresolution methods. It can make use of a cylindrical lens, a phase mask, a Spatial Light Modulator, a Deformable Mirror (DM) to encode depth on a spatial variation of the PSF along the Z axis. Among these techniques, we demonstrate that a DM-based approach – such as implemented in our MicAO-3DSR system - provides the best versatility/performance combination

Conference Presentation

Citation Download Citation

Fabrice Harms, Audrius Jasaitis, Cynthia Veilly, and Xavier Levecq "Best versatility/localization precision combination in single molecule localization microscopy enabled by adaptive optics: MicAO 3DSR", Proc. SPIE 11650, Single Molecule Spectroscopy and Superresolution Imaging XIV, 116500G (5 March 2021); https://doi.org/10.1117/12.2583292

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