Paper 13139-29
Paper 13139-29
Leveraging computational imaging for enhanced third-harmonic generation microscopy (Invited Paper)
19 August 2024 • 2:25 PM - 2:50 PM PDT | Conv. Ctr. Room 11B
Abstract
Third harmonic generation (THG) microscopy offers label-free imaging and enables three-dimensional imaging with inherently good optical depth-sectioning. Its utility is important for imaging and characterization of laser-induced changes in transparent materials. Here we develop a digital twin for THG microscopy that allows accurate simulation of experiments and provides insight into desired information such as the third-order nonlinear susceptibility tensor. It also enables accurate simulation of the instrument's point spread function (PSF), which is essential for relating THG measurements to the desired hidden quantities. Finally, we improve microscope throughput with adaptive optics, demonstrating how PSF engineering improves spatial probing, temporal efficiency, and information content, and comparing results between the real setup and its digital twin.
Presenter
Vasiliki Stergiopoulou
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Dr. Vasiliki Stergiopoulou joined the Galatea Lab, the Audiovisual Communication Laboratory, and the Imaging Center at EPFL as a postdoctoral researcher in May 2023. Prior to that, she received her master's degree in Electrical and Computer Engineering from the University of Patras in 2018 and completed her PhD in Automation, Signal, and Image Processing at the Université of Côte d'Azur in 2023. She has been a visiting researcher in the Biomedical Imaging Group at EPFL and the Cambridge Image Analysis Group for short periods. Her research focuses on image reconstruction and inverse problems in imaging, with a focus on applications in biomedical imaging and optics. In 2022, she received the Best Paper Runner-Up Award at the 19th IEEE International Symposium on Biomedical Imaging (ISBI).