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Proceedings Paper

Identification of non-neoplastic and neoplastic gastric polyps using multiphoton microscopy
Author(s): Shanghai Jiang; Deyong Kang; Meifang Xu; Xiaoqin Zhu; Shuangmu Zhuo; Jianxin Chen
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Paper Abstract

Gastric polyps can be broadly defined as luminal lesions projecting above the plane of the mucosal surface. They are generally divided into non-neoplastic and neoplastic polyps. Accurate diagnosis of neoplastic polyps is important because of their well-known relationship with gastric cancer. Multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) is one of the most important recent inventions in biological imaging. In this study, we used MPM to image the microstructure of gastric polyps, including fundic gland polyps, hyperplastic polyps, inflammatory fibroid polyps and adenomas, then compared with gold-standard hematoxylin- eosin(H-E)-stained histopathology. MPM images showed that different gastric polyps have different gland architecture and cell morphology. Dilated, elongated or branch-like hyperplastic polyps are arranged by columnar epithelial cells. Inflammatory fibroid polyps are composed of small, thin-walled blood vessels surrounded by short spindle cells. Fundic glands polyps are lined by parietal cells and chief cells, admixed with normal glands. Gastric adenomas are generally composed of tubules or villi of dysplastic epithelium, which usually show some degree of intestinal-type differentiation toward absorptive cells, goblet cells, endocrine cells. Our results demonstrated that MPM can be used to identify non- neoplastic and neoplastic gastric polyps without the need of any staining procedure.

Paper Details

Date Published: 11 December 2012
PDF: 6 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855311 (11 December 2012); doi: 10.1117/12.999842
Show Author Affiliations
Shanghai Jiang, Fujian Normal Univ. (China)
Deyong Kang, Fujian Medical Univ. (China)
Meifang Xu, Fujian Medical Univ. (China)
Xiaoqin Zhu, Fujian Normal Univ. (China)
Shuangmu Zhuo, Fujian Normal Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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