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Proceedings Paper

In-line retro-reflective polarizing contrast scope for translucent objects
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Paper Abstract

We propose a very-low-cost fixed interferential polarizing phase contrast scope suitable for the study of translucent objects. Our key design approach is relied on the arrangement of a circular polarizer sheet, a mirror, and a digital camera in a retro-reflective optical structure. The linear polarizer embedded in the circular polarizer sheet acts as both a polarization beam splitter and a polarization beam combiner. Meanwhile the quarter waveplate inside the circular polarizer sheet functions as a fixed phase plate but without narrowing the field of view of the digital camera. The retroreflective configuration amplifies the phase difference between the two orthogonal polarized optical beams twice, thus automatically creating an initial dark background. Experimental demonstration using an off-the-shelf digital microscope with built-in white light emitting diodes and a specified 400x maximum magnification, a circular polarizer sheet, and a mirror shows that onion cells and Steinernema Thailandense nematodes can be clearly observed with striking color, high contrast, and three-dimensional appearance.

Paper Details

Date Published: 21 November 2012
PDF: 7 pages
Proc. SPIE 8558, Optoelectronic Imaging and Multimedia Technology II, 85580G (21 November 2012);
Show Author Affiliations
Sarun Sumriddetchkajorn, National Science and Technology Development Agency (Thailand)

Published in SPIE Proceedings Vol. 8558:
Optoelectronic Imaging and Multimedia Technology II
Tsutomu Shimura; Guangyu Xu; Linmi Tao; Jesse Zheng, Editor(s)

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