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Proceedings Paper

Design of hybrid optical tweezers system for automated 3D micro manipulation
Author(s): Yoshio Tanaka; Shogo Tsutsui; Hiroyuki Kitajima
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Paper Abstract

In our previous paper, on dynamic handling of massive micro-bead arrays, we developed a hybrid optical tweezers system consisting of two multi-beam techniques: the GPC method and the galvano mirrors (GMs) scanning method. This system had high versatility for manipulating massive arrays, but arrays formed by its GM scanning tweezers could be handled only in a two-and-half dimensional (2.5D) working space. This limitation arose from the low bandwidth of the Z-axis manipulation due to the lens translation using a linear stage. For true 3D controlled manipulation of multiple micro-beads, in this paper, we redesign the GM scanning part of the previous hybrid system using an electrically focus-tunable lens with high bandwidth. The optical structure is linked to a commercially available microscope via its epifluorescence port. One set of optical tweezers based on the GPC uses the p-polarized beam, and the other set based on the GM scanning uses the s-polarized beam. In the results of the 3D manipulation experiment, the controlled rotation of five beads forming a pentagon and that of four beads forming a tetrahedron about arbitrary axes are demonstrated.

Paper Details

Date Published: 18 December 2012
PDF: 7 pages
Proc. SPIE 8550, Optical Systems Design 2012, 85501X (18 December 2012); doi: 10.1117/12.980463
Show Author Affiliations
Yoshio Tanaka, National Institute of Advanced Industrial Science and Technology (Japan)
Shogo Tsutsui, Kagawa Univ. (Japan)
Hiroyuki Kitajima, Kagawa Univ. (Japan)

Published in SPIE Proceedings Vol. 8550:
Optical Systems Design 2012
Laurent Mazuray; Daniel G. Smith; Jean-Luc M. Tissot; Tina E. Kidger; Frank Wyrowski; Stuart David; Rolf Wartmann; Jeffrey M. Raynor; Andrew P. Wood; Pablo Benítez; Andreas Erdmann; Marta C. de la Fuente, Editor(s)

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