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Proceedings Paper

Lab-on-a-bubble: direct and indirect assays with portable Raman instrumentation
Author(s): Keith Carron; Virginia Schmit; Brandon Scott; Richard Martoglio
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Paper Abstract

Lab-on-a-Bubble (LoB) is a new method for SERS (Surface Enhanced Raman Scattering) assays that combines separationand concentration of the assay results. A direct LoB assay is comprised of gold nanoparticles coupled directly to the ~30 μm diameter buoyant silica bubble. The direct LoB method was evaluated with cyanide and 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB). An indirect assay uses the same ~ 30 μm diameter buoyant silica bubble and a silica coated SERS reporter. Both the bubble and SERS reporter are coated with a coupling agent for the analyte. The assay measures the amount of SERS reporter coupled to the bubble through a sandwich created by the analyte. The couling agent could consist of an immunological coupling agent (antibody) or a nucleic acid coupling agent (single strand DNA). The indirect LoB method was examined with Cholera toxin (CT) and antibodies against the β subunit. An LOD of ~ 170 pptrillion was measured for cyanide and a limit of detection of 1100 ng was found for CT.

Instrumentation for the assay and a novel technique of dynamic SERS (DSERS) will also be discussed. The instrument is a small hand-held Raman device called the CBEx (Chemical Biological Explosive) with a novel raster system to detect heterogeneous or light sensitive materials. DSERS is a mathematical algorithm which eliminates background interference in SERS measurements with colloidal nanoparticles.

Paper Details

Date Published: 30 October 2012
PDF: 9 pages
Proc. SPIE 8546, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence VIII, 854606 (30 October 2012); doi: 10.1117/12.973767
Show Author Affiliations
Keith Carron, Univ. of Wyoming (United States)
Snowy Range Instruments (United States)
Virginia Schmit, Univ. of Wyoming (United States)
Brandon Scott, Univ. of Wyoming (United States)
Richard Martoglio, DePauw Univ (United States)

Published in SPIE Proceedings Vol. 8546:
Optics and Photonics for Counterterrorism, Crime Fighting, and Defence VIII
Colin Lewis; Douglas Burgess, Editor(s)

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