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Proceedings Paper

The Phase Transition Behaviour Of Multibilayer Preparations Of Free Lipid A, The Endotoxic Principle Of The Outer Membrane Of Gram-Negative Bacteria: A FT-IR Study
Author(s): D. Naumann; Ch. Schultz; H. Labischinski; H. Bradaczek
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Paper Abstract

FT-IR spectra of free lipid A, isolated from Escherichia coli containing C-14 and C-12 acyl chains have been recorded and tentative band assignments were undertaken. The highly complex phase transition behaviour of fully hydrated multibilayer preparations of lipid A has been investigated using temperature-induced frequency shifts, difference spectra and second derivatives of >CH2 asymmetric and symmetric stretching modes near 2918 and 2850 cm -1, respectively. Three "phase transitions" near 22, 34 and 41oC could be detected, the latter being the main transition and - most probably - the familiar B-u-transition. The high value of 41°C found for the main transition temperature may reflect the stability and permeation barrier function of this molecule within the outer membrane of gram-negative bacteria.

Paper Details

Date Published: 20 December 1985
PDF: 2 pages
Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); doi: 10.1117/12.970752
Show Author Affiliations
D. Naumann, Institute of Crystallography of the Free University of Berlin (Germany)
Ch. Schultz, Institute of Crystallography of the Free University of Berlin (Germany)
H. Labischinski, Robert Koch-Institute of the Federal Health Office of Germany (Germany)
H. Bradaczek, Institute of Crystallography of the Free University of Berlin (Germany)

Published in SPIE Proceedings Vol. 0553:
Fourier and Computerized Infrared Spectroscopy
David G. Cameron; Jeannette G. Grasselli, Editor(s)

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