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Proceedings Paper

Detection of Physiological Levels of Hormonal Steroids Labelled with Organometallic Markers by FT-IR Spectroscopy
Author(s): Ashraf A. Ismail; Ian S. Butler; Gerard Jaouen; Anne Vessieres; Sylvie Tondu; Siden Top
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Paper Abstract

Hormonal steroids such as 17α-estradiol derivatives, labelled with organometallic moieties, have been tested as markers for hormone receptor assay. Among the organometallic labels used were: Cr(C0)3 and Cr(C0)2(CS), which can be attached to the A ring of the steroid, and Co2(C0)6 and Mo2(7-15-05H5)(C0)4, which can be bonded to acetylenic groups in the 1713-position of the five-membered ring of the steroid. The new analytical approach is based on the FT-IR detection of the υ(CO) modes of the organometallic markers. The present estradiol-receptor detection level for 10,000-20,000 scans at 4 cm-1 resolution is about 100 fM, i.e., in the neighbourhood of physiological levels.

Paper Details

Date Published: 20 December 1985
PDF: 2 pages
Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); doi: 10.1117/12.970742
Show Author Affiliations
Ashraf A. Ismail, McGill University (Canada)
Ian S. Butler, McGill University (Canada)
Gerard Jaouen, Ecole Normale Superieure de Chimie de Paris (France)
Anne Vessieres, Ecole Normale Superieure de Chimie de Paris (France)
Sylvie Tondu, Ecole Normale Superieure de Chimie de Paris (France)
Siden Top, Ecole Normale Superieure de Chimie de Paris (France)

Published in SPIE Proceedings Vol. 0553:
Fourier and Computerized Infrared Spectroscopy
David G. Cameron; Jeannette G. Grasselli, Editor(s)

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