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Proceedings Paper

FT-IR Microscopy Of Biomineralization At 20µ Spatial Resolution
Author(s): Richard Mendelsohn; Alborz Hassankhani; Nancy Pleshko; Edward DiCarlo; Adele Boskey
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Paper Abstract

FT-IR microscopy offers certain advantages for the study of normal and pathological biomineralizing tissue samples. These include the identification and quantitation of the mineral phase and the determination of the secondary structure of the protein constituents. A drawback of the method is difficulty in interpretation of spectra from samples containing unknown quantities of unknown constituents. We have circumvented this problem by studying the main components of mineralizing tissue, collagen (95% of the protein phase) and hydroxyapatite (main mineral constituent) whose strongest IR bands absorb in relatively clean spectral regions. Samples were prepared for IR microscopy by surgical removal of tissue, fixation in formalin or ethanol, embedding in PMMA or epoxy, and the microtoming of thin (5-8μ) sections. Bulk embedding agent was mostly removed with methyl acetate. The sample was squeezed between BaF2 windows for IR studies. Any remaining PMMA or epoxy was removed by spectral subtraction. To evaluate the utility of FT-IR microscopy for study of biomineralization, two systems were examined:

Paper Details

Date Published: 1 December 1989
PDF: 2 pages
Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); doi: 10.1117/12.969521
Show Author Affiliations
Richard Mendelsohn, Rutgers University (United States)
Alborz Hassankhani, Rutgers University (United States)
Nancy Pleshko, Rutgers University (United States)
Edward DiCarlo, Cornell University Medical College and the Hospital for Special Surgery (United States)
Adele Boskey, Cornell University Medical College and the Hospital for Special Surgery (United States)

Published in SPIE Proceedings Vol. 1145:
7th Intl Conf on Fourier Transform Spectroscopy
David G. Cameron, Editor(s)

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