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Proceedings Paper

Three-Dimensional Reconstruction Of Noisy Confocal Scanning Microscope Images
Author(s): Jose-Angel Conchello; Eric W. Hansen
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Paper Abstract

Confocal scanning microscopes have improved depth discrimination over conventional microscopes. The out-of-focus contributions in a through-focus series of images are greatly reduced by the confocal geometry but not completely removed. Simulations have shown that posteriori iterative constrained deconvolution can produce depth discrimination equal to the lateral diffraction limit. However, such deterministic algorithms perform poorly when the optical sections are noisy, such as occurs in ultralow-light fluorescence microscopy. In this paper we present a stochastic approach to 3-D reconstruction and discuss the relationship of the microscope's imaging properties to the postprocessing task.

Paper Details

Date Published: 22 December 1989
PDF: 7 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962710
Show Author Affiliations
Jose-Angel Conchello, Dartmouth College (United States)
Eric W. Hansen, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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