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Proceedings Paper

A Variable Intensity Electroluminescence Standard For Video Microscopy
Author(s): Dennis J. O'Kane; John E. Wampler
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Paper Abstract

Electroluminescent lamps can be used as a convenient stable source of luminescence over at least a three thousand-fold range of light intensity. The electroluminescence (EL) output increases with increasing applied voltage. Using a Quantex 3 x 3 inch EL lamp with a green-emitting phosphor, we found that as little as 10 VAC can be used to elicit EL and that the EL emission spectrum remained constant over a wide range of applied voltages (λ re- 508 nm; FWHM = 75 nm). Resolution measurements can be made under conditions of high or low light intensities by placing a resolution target over the EL lamp and manipulating the voltage, or by using a neutral density filter to achieve the desired light intensity, or by combining both procedures to achieve the desired light intensity and image content. The absolute EL flux, per unit area of the lamp, can be determined using the ferrioxalate chemical actinometer, corrected for light transmission and the corrected emission spectrum of the lamp. The EL lamp can thus also be used as a tertiary radiometric standard for quantification, as well as for resolution testing.

Paper Details

Date Published: 22 December 1989
PDF: 11 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962700
Show Author Affiliations
Dennis J. O'Kane, University of Georgia (United States)
John E. Wampler, University of Georgia (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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