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Proceedings Paper

EDKC Selectively Photosensitizes Respiration Of Malignant Cells In Vitro.
Author(s): G. Ara; L. Lewandowski; A. R. Oseroff
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Paper Abstract

We have shown previously that the cationic cyanine dye, EDKC, which photosensitizes malignant cells in vitro and in vivo, blocks respiration in isolated murine liver mitochondria by preferentially inactivating Complex I mediated electron transport. The present work was directed towards examining whether the killing of malignant cells with EDKC and visible radiation was a result of irreparable damage to mitochondria of these cells. We report that EDKC selectively photosensitizes respiration of squamous carcinoma and melanoma cells in vitro. At 0.1 uM, the dye inhibited basal respiration, however, an uncoupler, FCCP, reverted the respiration rate to the control uncoupled rate. Furthermore, the basal respiration of control or EDKC treated cells was totally inhibited by oligomycin, an inhibitor of FoFiATPase, indicating that the respiration was coupled to phosphorylation. After irradiation with 700 + 20 nm light (14 J/cm2) in the presence of 0.1 uM dye, basal respiration was slightly enhanced compared to that of the dye treated cells, however, FCCP-stimulated respiration was drastically lowered. In addition, only 10-20% of the basal respiration was blocked by oligomycin, indicating that a major part of respiration was no longer coupled to phosphorylation. Furthermore, almost no recovery of the mitochondria! respiratory function was detected 6 hr after treatment. Respiration of an untransformed monkey kidney cell line (CV-1) was only minimally affected by ten fold higher dye concentration (1 uM) and 14 J/cm2 radiation.

Paper Details

Date Published: 17 March 1989
PDF: 8 pages
Proc. SPIE 0997, Advances in Photochemotherapy, (17 March 1989); doi: 10.1117/12.960186
Show Author Affiliations
G. Ara, Tufts-New England Medical Center (United States)
L. Lewandowski, Tufts-New England Medical Center (United States)
A. R. Oseroff, Tufts-New England Medical Center (United States)

Published in SPIE Proceedings Vol. 0997:
Advances in Photochemotherapy
Tayyaba Hasan, Editor(s)

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