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Proceedings Paper

In Vitro Fluorescence Studies With Hematoporphyrin Derivative
Author(s): Janine R. Shulok; Margaret H. Wade; Chi-Wei Lin
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Paper Abstract

The translocation and localization pattern of hematoporphyrin derivative (HpD) in human bladder tumor cells (MGH-U1) in culture was evaluated using fluorescence microscopy and interactive laser cytometry. The qualitative and quantitative fluorescence labelling patterns of HpD were compared with other fluorescent dyes specific for subcellular localization sites. Labelling of MGH-U1 cells with HpD initially showed fluorescence at the cell membrane. Later, diffuse perinuclear fluorescence was evident in these cells. This fluorescent staining pattern did not appear to be site-specific to either the mitochondria or endoplasmic reticulum but appeared to be generally distibuted to the cytomembranous structures of the cell. Lateral diffusion measurements indicated that the fluorescent components of HpD were bound to cellular lipids. These results suggest that HpD may localize in cells in vitro by a spontaneous diffusion process and be distributed to lipophilic sites within the cell.

Paper Details

Date Published: 17 March 1989
PDF: 5 pages
Proc. SPIE 0997, Advances in Photochemotherapy, (17 March 1989); doi: 10.1117/12.960180
Show Author Affiliations
Janine R. Shulok, Harvard Medical School (United States)
Margaret H. Wade, Harvard Medical School (United States)
Chi-Wei Lin, Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 0997:
Advances in Photochemotherapy
Tayyaba Hasan, Editor(s)

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