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Proceedings Paper

The Induction By Laser Light Of The In Vitro Flowering Of Cichorium intybus L. (Long day plant)
Author(s): Pierre Paulet; Philippe Badila
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Paper Abstract

Flower inflorescences can be induced directly on root explants from the late variety of chicory cultivated in vitro. In vitro explants require cold treatment and long days to produce flowers. Initial studies on the level of endogenous phenolics and cytokinins in root explants during chilling treatment showed important changes and similar studies were desirable on the effect of long day treatments. However, during long days it is quite difficult to know if variations in these compounds depend on the trophic effect of light, i.e., photosynthesis or on the photoperiodic effect, i.e., tautomeric change in the structure of phytochrome. To separate photoperiodic effects from photosynthetic effects, explants were maintained in short days (10 hours light) and given photoperiodic induction by very brief irradiation with a dye laser producing 660 nn light of 150 mw/cm2. Controls in short days do not flower but explants irradiated for only 0.5 minute by the laser produced flowers. Significant changes in dicaffeylquinic acids (isochlorogenic acid) were obtained after only 7 seconds irradiation which are also correlated with flower induction. The results suggest that laser irradiation may prove important in agricultural practice.

Paper Details

Date Published: 29 May 1980
PDF: 3 pages
Proc. SPIE 0211, Optics and Photonics Applied to Medicine, (29 May 1980); doi: 10.1117/12.958344
Show Author Affiliations
Pierre Paulet, University of Orleans (France)
Philippe Badila, University of Orleans (France)

Published in SPIE Proceedings Vol. 0211:
Optics and Photonics Applied to Medicine
Michel H. Grosmann; Patrick Meyrueis, Editor(s)

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