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Proceedings Paper

Imaging System For Correlating Fluorescence Cell Measurements In Flow
Author(s): David B. Kay; James L. Cambier; Leon L. Wheeless
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Paper Abstract

A high sensitivity camera and imaging system are employed in a special purpose flow cytofluorometer for extraction of fluorescence images of cells and objects in flow. Each image is uniquely correlated with a fluorescence profile or "slit-scan" contour of the cell also derived in flow. Contours are computer analyzed to extract low resolution morphologic information. Optical considerations for imaging in flow and results obtained from the imaging system are presented.

Paper Details

Date Published: 21 November 1977
PDF: 7 pages
Proc. SPIE 0126, Clever Optics: Innovative Applications of Optics, (21 November 1977); doi: 10.1117/12.955901
Show Author Affiliations
David B. Kay, University of Rochester (United States)
James L. Cambier, University of Rochester (United States)
Leon L. Wheeless, University of Rochester (United States)

Published in SPIE Proceedings Vol. 0126:
Clever Optics: Innovative Applications of Optics
N. Balasubramanian; James C. Wyant, Editor(s)

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