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Proceedings Paper

A Spectral Method For The Rapid Detection And Identification Of Human Viruses
Author(s): Alan Pronovost; Pei Chang; Wilfred H. Nelson
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Paper Abstract

Poliovirus type I, strain LSc2ab, has been grown in HeLa cell cultures and purified by means of a five-step process involving cell debris removal, virus precipitation with polyethylene glycol 6000, ultrasedimentation into a discontinuous CsCl step gradient, chloroform extraction in the hypertonic state, and isopycnic density gradient centrifugation. Virus concentration of 1.5-3.0 mg/ml in 20 μliter samples was achieved by volume reduction. Laser-Raman spectra of the purified samples exhibit characteristic lines which can be assigned to the viral coat protein and the RNA. Because characteristic spectral "fingerprints" can be obtained in a matter of minutes, it may be possible to develop a method of rapid viral analysis based upon molecular spectroscopy. Our study shows for the first time that a human virus can be identified by Raman spectroscopy.

Paper Details

Date Published: 20 January 1977
PDF: 6 pages
Proc. SPIE 0089, Applications of Optics in Medicine and Biology, (20 January 1977); doi: 10.1117/12.955028
Show Author Affiliations
Alan Pronovost, University of Rhode Island (United States)
Pei Chang, University of Rhode Island (United States)
Wilfred H. Nelson, University of Rhode Island (United States)

Published in SPIE Proceedings Vol. 0089:
Applications of Optics in Medicine and Biology
Robin E. Herron, Editor(s)

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