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Proceedings Paper

Investigation Of DNA's By A New Pseudo-Raman Technique
Author(s): J. P. Biscar; N. Kollias; P . D. Bear
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Paper Abstract

The Raman spectrum of long chain biological polymers displays a broad band which is distinct from conventional molecular Raman lines. A hypothesis based on a new Electromagnetic Molecular Electronic Resonance has been advanced to explain it. The resolved broad band data of Poly-L-Glutamic acid and Bovine Serum Albumin coincide with the predictions of this theory. The technique has been extended to the analysis of double stranded DNA's. . Spectra obtained from T7 and A viruses show that there is an Electromagnetic Molecular Electronic Resonance taking place along 1 orbital chains between coupled bases. This provides the first experimental proof that there is a IT orbital coupling from base to base in double stranded DNA's. A base dislocation along the DNA is detected in the spectrum. An important feature of this new technique is that it does identify a virus DNA, and measures its length, when the latter is still inside its protein coat.

Paper Details

Date Published: 1 May 1974
PDF: 12 pages
Proc. SPIE 0043, Application of Optical Instrumentation in Medicine II, (1 May 1974); doi: 10.1117/12.953901
Show Author Affiliations
J. P. Biscar, The University of Wyoming (United States)
N. Kollias, The University of Wyoming (United States)
P . D. Bear, The University of Wyoming (United States)

Published in SPIE Proceedings Vol. 0043:
Application of Optical Instrumentation in Medicine II
William R. Hendee; William C. Zarnstorff, Editor(s)

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