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Proceedings Paper

Imaging Flow Cytometer
Author(s): Chi Keung Yeung; Peter Nickolls; Mel Clarke; Sim Heng Ong; David Horne
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Paper Abstract

An imaging flow cytometer is described in this paper. It possesses the high cell processing rate of a flow cytometer and the high image resolution of an automated microscope. It is a flow cytometer to which has been added a cell detector and velocity measuring circuit and a microscope objective lens that focuses cell illuminated by a laser onto a two-dimensional charge-coupled device (CCD) array. Optical sensing and imaging are carried out in a water filled chamber to reduce optical abberration. The time-delay and integration (TDI) technique is utilized for image acquisition. Image processing and classification is to be carried out in real time by a n-channel, metal-oxide-semicoductor (nMOS) parallel processor array. This imaging flow cytometer can acquires image at a flow rate of 0.5 m/s and in the future will be capable of analysing and sorting cells at high speed on the basis of simultaneous morphology as well as cytochemistry and immunology.

Paper Details

Date Published: 13 June 1989
PDF: 6 pages
Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); doi: 10.1117/12.951894
Show Author Affiliations
Chi Keung Yeung, University of Sydney (Australia)
Peter Nickolls, University of Sydney (Australia)
Mel Clarke, University of Sydney (Australia)
Sim Heng Ong, University of Sydney (Australia)
David Horne, University of Sydney (Australia)

Published in SPIE Proceedings Vol. 1063:
New Technologies in Cytometry
Gary C. Salzman, Editor(s)

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