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Proceedings Paper • Open Access

Synchronous Fluorescence Studies Of Anthracycline Anti-Tumor Drugs
Author(s): Bruce J. Tromberg; Thomas G. Burke; James H. Doroshow; Michael W. Berns

Paper Abstract

Synchronous fluorescence (SF) is used to study structurally and spectrally similar anthracycline anti-tumor drugs in biological fluids and model membranes. SF bandwidths at half maxima are typically 20-24 nm versus 75-80 nm for unresolved spectra acquired during conventional scans. This substantial band-narrowing effect permits excellent resolution between Adriamycin and an interferant, 5- iminodaunorubicin, in a two-component mixture. SF detection limits for Adriamycin are 7 x 10-7 M in serum and 1.5 x 10-7 M in buffered aqueous solution. Additional studies of structurally homologous drugs in liposomes and organic solvents indicate that SF may be useful in characterizing the influence of substituent groups on relative membrane permeation depth.

Paper Details

Date Published: 17 May 1989
PDF: 8 pages
Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951552
Show Author Affiliations
Bruce J. Tromberg, University of California (United States)
Thomas G. Burke, City of Hope National Medical Center (United States)
James H. Doroshow, City of Hope National Medical Center (United States)
Michael W. Berns, University of California (United States)

Published in SPIE Proceedings Vol. 1054:
Fluorescence Detection III
E. Roland Menzel, Editor(s)

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