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Proceedings Paper

Standards For Corrected Fluorescence Spectra
Author(s): Ambler Thompson; Kenneth L. Eckerle
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Paper Abstract

A set of four fluorescent standards has been produced and calibrated at the National Institute of Standards and Technology (NIST) for corrected relative spectral emission over the wavelength range from 400 to 740 nm. This new Standard Reference Material (SRM) 1931 has been produced in the form of sintered mixtures of inorganic phosphors and polytetrafluorethylene (PTFE) powder for spectral response calibration of spectrofluorimeters used in biology, medicine and studies of molecular structure. They provide a means for correcting spectrofluorimeter errors due to wavelength dependencies of optics, monochromators, and detectors, thus permitting meaningful intercomparison of results obtained in different laboratories.

Paper Details

Date Published: 17 May 1989
PDF: 6 pages
Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951539
Show Author Affiliations
Ambler Thompson, National Institute of Standards and Technology (United States)
Kenneth L. Eckerle, National Institute of Standards and Technology (United States)

Published in SPIE Proceedings Vol. 1054:
Fluorescence Detection III
E. Roland Menzel, Editor(s)

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