The spectrum acquired at the sample phototnultiplier tube of a fluorescence spectrophotometer is a product of the sample spectrum and the instrument function. The determination of the instrument function and its removal from the acquired spectrum is often critical to the accurate determination of the physical properties of the sample. Methods are discussed for the determination and removal of the instrument function from excitation and emission spectra. Methods considered include quantum counters and ratio circuits for excitation correction, and emission correction against calibrated excitation systems, calibrated tungsten lamps, and NBS standard quinine sulfate.

Date Published: 17 May 1989
PDF: 12 pages
Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); doi: 10.1117/12.951538

Published in SPIE Proceedings Vol. 1054:
Fluorescence Detection III
E. Roland Menzel, Editor(s)