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Proceedings Paper

In Vivo Confocal Imaging Of The Eye Using Tandem Scanning Confocal Microscopy (TSCM):
Author(s): James V. Jester; H. Dwight Cavanagh; Michael A. Lemp
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Paper Abstract

The tandem scanning reflected light microscope (TSRLM) is a confocal light microscope which has the capability of looking into living tissue and obtaining high resolution, high magnification images of cellular structure. TSRLM can be used to study living tissue such as all layers of the corneal epithelium including basal epithelial cells, keratocytes, nerves, inflammatory cells, bacteria, and corneal endothelium. For the first time in vision research, real-time, in vivo, microscopic images of normal and pathologic tissues can be obtained from human or animal eyes using the TSRLM. Compared to other methods of vital microscopy, TSRLM has no present rival. Specifically, TSRLM will: (1) Allow the hitopathologic analysis of living eyes, in vivo, over multiple observation periods without the need for tissue fixation and/or processing; (2) Assist in the acquisition and analysis of histopathologic images from human eyes, in vivo, in corneal disease; and (3) Greatly reduce the need for large numbers of animals in the histopathologic evaluation of experimental corneal disease and surgical procedures.

Paper Details

Date Published: 9 February 1989
PDF: 5 pages
Proc. SPIE 1028, Scanning Imaging, (9 February 1989); doi: 10.1117/12.950323
Show Author Affiliations
James V. Jester, Georgetown University (United States)
H. Dwight Cavanagh, Georgetown University (United States)
Michael A. Lemp, Georgetown University (United States)

Published in SPIE Proceedings Vol. 1028:
Scanning Imaging
Tony Wilson, Editor(s)

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