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Proceedings Paper

Modelling Of 3-D Confocal Imaging At High Numerical Aperture In Fluorescence
Author(s): H. T. M. van der Voort; G. J. Brakenhoff
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Paper Abstract

The imaging properties of a model high aperture fluorescence confocal microscope are studied by way of numerical methods. The model consists of an aberration-free high aperture objective illuminated by a monochromatic linear or circular polarized light source, and a detection system equipped with a finite sized pinhole. The computation of the intensity distribution near the confocal plane is based on electromagnetic diffraction theory. We study the 3-D imaging properties of the model by examining the images of three different objects; points, lines and planes. We calculate the resolving power for these objects as a function of their orientation and the size of the detector pinhole. The results indicate the necessity for image analysis schemes to take these effects into account.

Paper Details

Date Published: 9 February 1989
PDF: 6 pages
Proc. SPIE 1028, Scanning Imaging, (9 February 1989); doi: 10.1117/12.950303
Show Author Affiliations
H. T. M. van der Voort, University of Amsterdam (The Netherlands)
G. J. Brakenhoff, University of Amsterdam (The Netherlands)

Published in SPIE Proceedings Vol. 1028:
Scanning Imaging
Tony Wilson, Editor(s)

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