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Proceedings Paper

Spherical Aberration In Confocal Microscopy
Author(s): T. Hellmuth; P. Seidel; A. Siegel
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Paper Abstract

Scanning confocal microscopy has become an important tool in the field of optical sectioning and 3D-reconstruction of biological objects. Usually these samples are embedded in dispersive media, introducing depth dependent spherical aberration. Depth and lateral resolution are therefore impaired. We discuss this problem theoretically and show experimental results.

Paper Details

Date Published: 9 February 1989
PDF: 5 pages
Proc. SPIE 1028, Scanning Imaging, (9 February 1989); doi: 10.1117/12.950300
Show Author Affiliations
T. Hellmuth, Carl Zeiss (Federal Republic of Germany)
P. Seidel, Carl Zeiss (Federal Republic of Germany)
A. Siegel, Carl Zeiss (Federal Republic of Germany)

Published in SPIE Proceedings Vol. 1028:
Scanning Imaging
Tony Wilson, Editor(s)

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