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Proceedings Paper

Optical Microscopic Tomography
Author(s): Satoshi Kawata; Yusuke Touki; Shigeo Minami
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Paper Abstract

A new principle to obtain sectional images of a thick specimen by a conventional optical microscope without physically slicing is developed. The optical microscope with an off-axis pupil is used to project a 3-D distribution of the specimen in various directions within the numerical aperture of the objective lens. Since the system is the strictly angularly-limited one, a strong constraint is needed to reconstruct the 3-D structure from the projections. The conjugate gradient method with the object boundary constraint which is a priori known meets this problem. Experimental results with a biological sample verify the capability of the proposed method as optical tomography of microscopic size.

Paper Details

Date Published: 25 October 1985
PDF: 6 pages
Proc. SPIE 0558, Inverse Optics II, (25 October 1985); doi: 10.1117/12.949566
Show Author Affiliations
Satoshi Kawata, Osaka University (Japan)
Yusuke Touki, Osaka University (Japan)
Shigeo Minami, Osaka University (Japan)

Published in SPIE Proceedings Vol. 0558:
Inverse Optics II
Richard H.T. Bates; Anthony J. Devaney, Editor(s)

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