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Proceedings Paper

Quantum dots induce charge-specific amyloid-like fibrillation of insulin at physiological conditions
Author(s): Alyona Sukhanova; Simon Poly; Anton Shemetov; Igor R. Nabiev
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Paper Abstract

Agglomeration of some proteins may give rise to aggregates that have been identified as the main cause of amyloid diseases. For example, fibrillation of insulin is related to diabetes mellitus. Quantum dots (QDs) are of special interest as tagging agents for diagnostic and therapeutic studies due to their broad absorption spectra, narrow emission spectra, and high photostability. In this study, PEGylated CdSe/ZnS QDs have been shown to induce the formation of amyloid-like fibrils of human insulin under physiological conditions, this process being dependent on the variation of the surface charge of the nanoparticles (NPs) used. Circular dichroism (CD), protein secondary structure analysis, thioflavin T (ThT) fluorescence assay, and the dynamic light scattering (DLS) technique have been used for comparative analysis of different stages of the fibrillation process. In particular, insulin secondary structure remodelling accompanied by a considerable increase in the rate of amyloid fiber formation have been observed after insulin was mixed with PEGylated QDs. Nanoparticles may significantly influence the rate of protein fibrillation and induce new mechanisms of amyloid diseases, as well as offer opportunities for their treatment.

Paper Details

Date Published: 24 October 2012
PDF: 9 pages
Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85485F (24 October 2012); doi: 10.1117/12.946606
Show Author Affiliations
Alyona Sukhanova, Trinity College Dublin (Ireland)
Moscow Engineering Physics Institute (Russian Federation)
Simon Poly, CIC nanoGUNE Consolider (Spain)
Anton Shemetov, Moscow Engineering Physics Institute (Russian Federation)
Igor R. Nabiev, Trinity College Dublin (Ireland)
Moscow Engineering Physics Institute (Russian Federation)

Published in SPIE Proceedings Vol. 8548:
Nanosystems in Engineering and Medicine
Sang H. Choi; Jin-Ho Choy; Uhn Lee M.D.; Vijay K. Varadan, Editor(s)

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