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Proceedings Paper

Using DNA looping to measure sequence dependent DNA elasticity
Author(s): Alan Kandinov; Krishnan Raghunathan; Jens-Christian Meiners
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Paper Abstract

We are using tethered particle motion (TPM) microscopy to observe protein-mediated DNA looping in the lactose repressor system in DNA constructs with varying AT / CG content. We use these data to determine the persistence length of the DNA as a function of its sequence content and compare the data to direct micromechanical measurements with constant-force axial optical tweezers. The data from the TPM experiments show a much smaller sequence effect on the persistence length than the optical tweezers experiments.

Paper Details

Date Published: 10 October 2012
PDF: 9 pages
Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 845816 (10 October 2012); doi: 10.1117/12.945930
Show Author Affiliations
Alan Kandinov, Univ. of Michigan (United States)
Krishnan Raghunathan, Univ. of Michigan (United States)
Jens-Christian Meiners, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8458:
Optical Trapping and Optical Micromanipulation IX
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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