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Proceedings Paper

Immobilization Of Unicellular Marine Organisms For Optical Characterization: A New Method
Author(s): K Lofftus; A J Hunt; M S Quinby-Hunt; F Livolant; M F Maestre
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Paper Abstract

A new method has been developed to study the light scattering properties of single, immobilized unicellular marine organisms. Cells of the dinoflagellate Prorocentrum micans were fixed1 and immobilized by mixing them into a solution that formed a transparent gel. The residual Rayleigh scattering caused by the gel was effectively eliminated by substituting a second liquid into the gel to match the index of refraction of the gel network, thereby producing a transparent support medium. Scattering measurements of the elements of the Mueller matrix were performed on a single Prorocentrum micans cell using a polarization-modulation scanning nephelometer.2 The angular dependence of Su, and normalized S12, S22, S33, S34, S13, and S14 are presented. The structure in all elements is very rich, cell orientation dependent, and completely reproducible. The measurements also revealed significantly non-zero values for all 16 elements of the scattering matrix and the non-equivalence of cross-diagonal elements. A striking feature of the measurements is the large peak values of S14 for a single immobilized cell. In recent years S14 has generally been reported to be zero for sea water and very small for laboratory systems. Measurements averaged over an ensemble of cells showed lower, but distinctly non-zero values in accord with earlier predictions.3

Paper Details

Date Published: 12 August 1988
PDF: 10 pages
Proc. SPIE 0925, Ocean Optics IX, (12 August 1988); doi: 10.1117/12.945741
Show Author Affiliations
K Lofftus, Lawrence Berkeley Laboratory (United States)
A J Hunt, Lawrence Berkeley Laboratory (United States)
M S Quinby-Hunt, University of California (United States)
F Livolant, Lawrence Berkeley Laboratory (United States)
M F Maestre, Lawrence Berkeley Laboratory (United States)

Published in SPIE Proceedings Vol. 0925:
Ocean Optics IX
Marvin A. Blizard, Editor(s)

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