Several workers have recently shown that time-resolved methods (or their frequency domain analogs) can offer significant improvements in the sensitivity of fluorescence immunoassays. However, the fluorescent labels most often used, chelates of terbium and europium have some drawbacks. We have developed a new fluorescent label with improved characteristics for such immunoassays, including excitation in the visible by laser sources, no requirement for a separate "enhancer solution", good time resolution characteristics, and the capability to be passively concentrated out of solution in front of the detector optics.

Date Published: 24 June 1988
PDF: 8 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945423

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)