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Proceedings Paper

Fluorescence Depolarization Studies Of Molecular Dynamics Using Pulsed Synchrotron Radiation: Applications To Lipid Vesicles And Oriented Multibilayers
Author(s): Herman van Langen; Gijs van Ginkel; Yehudi K Levine
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Paper Abstract

Analysis of time-resolved fluorescence anisotropy measurements on TMA-DPH molecules in POPC vesicles in terms of the rotational diffusion model reveals two distinct X2r minima which are statistically equivalent. The ambiguity in the interpretation is resolved by carrying out angle-resolved fluorescence depolarization experiments on planar POPC multibilayers in the time domain. It is shown that the order parameters of the probe molecules are higher in the multibilayers than in the vesicles. Furthermore the reorientational motion of the probes is considerably slower in the multibilayer sample. These differences are ascribed to the effects of the curvature and hydration of the bilayers in the vesicle systems.

Paper Details

Date Published: 24 June 1988
PDF: 12 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945417
Show Author Affiliations
Herman van Langen, University of Utrecht (The Netherlands)
Gijs van Ginkel, University of Utrecht (The Netherlands)
Yehudi K Levine, University of Utrecht (The Netherlands)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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