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Proceedings Paper

Resolution Of Overlapping Spectra Using Frequency-Domain Fluorometry
Author(s): Beniamino F Barbieri; Fabio De Piccoli; Enrico Gratton
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Paper Abstract

Commercial high-resolution phase and modulation fluorometers allow real time resolution of excitation and emission spectra of fluorophores with different lifetimes in a mixture. The technique can also be used for the separation of fluorescence and phosphorescence spectra of the fluorophore.

Paper Details

Date Published: 24 June 1988
PDF: 4 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945415
Show Author Affiliations
Beniamino F Barbieri, I.S.S. Inc. (United States)
Fabio De Piccoli, I.S.S. Inc (United States)
Enrico Gratton, University of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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