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Proceedings Paper

Multifrequency Phase-Resolution For Total Luminescence Spectroscopy
Author(s): Linda B McGown; David W Millican
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Paper Abstract

Multifrequency phase-resolved fluorescence spectroscopy is used to generate a multidimensional data array in which phase-resolved fluorescence intensity is measured as a function of emission wavelength and excitation wavelength at a series of excitation modulation frequencies. The dependence of the phase-resolved fluorescence intensity on fluorescence lifetime, modulation frequency and detector phase angle setting is examined. A model two-component system of benzo(k)fluoranthene and benzo(b)fluoranthene has been used to demonsrate the ability of phase-resolved fluorescence spectroscopy to selectively enhance the spectral contribution of a component as a function of fluorescence lifetime in total luminescence spectroscopyl, and is also discussed here.

Paper Details

Date Published: 24 June 1988
PDF: 6 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945414
Show Author Affiliations
Linda B McGown, Duke University (United States)
David W Millican, Duke University (United States)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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