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Proceedings Paper

Development Of A Time-Resolved Microfluorimeter With A Synchroscan Streak Camera And Its Application To Studies Of Cell Membranes
Author(s): Akihiro Kusurni; Akihiko Tsuji; Masayuki Murata; Yasushi Sako; Akiyasu C Yoshizawa; Tsuyoshi Hayakawa; Shun-ichi Ohnishi
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Paper Abstract

A time-resolved microfluorimeter with a synchroscan streak camera, which can be extended to a fluorescence lifetime imaging microscope (FLIM), has been developed for the use in biomedical research and clinical diagnosis. The schematic diagram of the instrument is given in Figure 1. The key idea for this instrument is to acquire molecular information on the structure of interest observed under the fluorescence microscope by using time-resolved fluorescence spectroscopy. The fluorescence lifetime is probably the only physical observable, the absolute value of which can be reliably determined in fluorescence microscopy.

Paper Details

Date Published: 24 June 1988
PDF: 2 pages
Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945412
Show Author Affiliations
Akihiro Kusurni, Kyoto University (Japan)
Akihiko Tsuji, Hamamatsu Photonics (Japan)
Masayuki Murata, Kyoto University (Japan)
Yasushi Sako, Kyoto University (Japan)
Akiyasu C Yoshizawa, Kyoto University (Japan)
Tsuyoshi Hayakawa, Hamamatsu Photonics (Japan)
Shun-ichi Ohnishi, Kyoto University (Japan)

Published in SPIE Proceedings Vol. 0909:
Time-Resolved Laser Spectroscopy in Biochemistry
Joseph R. Lakowicz, Editor(s)

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